Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.14.92 extracted from

  • Reddy, C.C.; Vaidyanathan, C.S.
    Purification and properties of benzoate-4-hydroxylase from a soil pseudomonad (1976), Arch. Biochem. Biophys., 177, 488-498.
    View publication on PubMed
Search for more literature for 1.14.14.92

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol hydroxylation is not supported when NADH, NADPH, dithiothreitol, 2-mercaptoethanol and ascorbic acid are added alone to the reaction mixture, stimulation when added together with tetrahydropteridine Pseudomonas sp.
ascorbic acid hydroxylation is not supported when NADH, NADPH, dithiothreitol, 2-mercaptoethanol and ascorbic acid are added alone to the reaction mixture, stimulation when added together with tetrahydropteridine Pseudomonas sp.
dithiothreitol hydroxylation is not supported when NADH, NADPH, dithiothreitol, 2-mercaptoethanol and ascorbic acid are added alone to the reaction mixture, stimulation when added together with tetrahydropteridine Pseudomonas sp.

General Stability

General Stability Organism
freezing and thawing has no effect Pseudomonas sp.
quite stable in presence of benzoate Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
4-Chlorobenzoate
-
 Pseudomonas sp.
4-Methylbenzoate
-
 Pseudomonas sp.
4-nitrobenzoate 38% inhibition Pseudomonas sp.
benzaldehyde
-
 Pseudomonas sp.
benzoate methyl ester slight inhibition Pseudomonas sp.
Cu2+ 0.005 mM, 50% inhibition Pseudomonas sp.
Hg2+ 0.005 mM, 70% inhibition Pseudomonas sp.
m-Hydroxybenzoate 12% inhibition Pseudomonas sp.
salicylate 20% inhibition Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.045
-
 NADH
-
 Pseudomonas sp.
0.045
-
 6,7-dimethyltetrahydropterin
-
 Pseudomonas sp.
0.3
-
 benzoate
-
 Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ preincubation for 10 min in the presence of benzoate results in maximal activity Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000
-
 gel filtration Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
protamine sulfate, heat treatment, calcium phosphate gel, ammonium sulfate, DEAE-Sephadex, Sephadex G-150 Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
33.92
-
-
 Pseudomonas sp.

Storage Stability

Storage Stability Organism
0-4°C, enzyme concentration 0.050 mg/ml, pH 6.0-7.5, 15 days, no loss of activity Pseudomonas sp.
0-4°C, pH 7.2-8.0, Tris-HCl buffer, 24 h, 50% loss of activity Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-chlorobenzoate + [reduced NADPH-hemoprotein reductase] + O2
-
 Pseudomonas sp. ?
-
 ?
4-methylbenzoate + [reduced NADPH-hemoprotein reductase] + O2
-
 Pseudomonas sp. ?
-
 ?
benzoate + 6,7-dimethyltetrahydropterin + O2
-
 Pseudomonas sp. 4-hydroxybenzoate + oxidized 6,7-dimethyltetrahydropterin + H2O
-
 r
benzoate + NADH + H+ + O2
-
 Pseudomonas sp. 4-hydroxybenzoate + NAD+ + H2O
-
 r
benzoate + [reduced NADPH-hemoprotein reductase] + O2
-
 Pseudomonas sp. 4-hydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
34
-
-
 Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
 Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8.2 approx. 30% of maximal activity at pH 5.5, approx. 35% of maximal activity at pH 8.2 Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
tetrahydropteridine essential for activity, hydroxylation is not supported when NADH, NADPH, dithiothreitol, 2-mercaptoethanol and ascorbic acid are added alone to the reaction mixture, stimulation when added together with tetrahydropteridine Pseudomonas sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.75
-
 4-nitrobenzoate
-
 Pseudomonas sp.