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Literature summary for 1.14.14.90 extracted from

  • Chen, J.; Yuan, H.; Zhang, L.; Pan, H.; Xu, R.; Zhong, Y.; Chen, J.; Nan, P.
    Cloning, expression and purification of isoflavone-2'-hydroxylase from Astragalus membranaceus Bge. Var. mongolicus (Bge.) Hsiao (2015), Protein Expr. Purif., 107, 83-89 .
    View publication on PubMed
Search for more literature for 1.14.14.90

Cloned(Commentary)

Cloned (Comment) Organism
gene AmI2'H or CYP81E42, DNA and amino acid sequence determination and analysis, recombinant expression of truncated enzyme, lacking the N-terminal membrane binding motif, in Escherichia coli strain BL21(DE3). Expression of the enzyme without removal of the transmembrane domain or insertion of the hydrophilic peptide AKKTSSKGKL is unsuccessful Astragalus membranaceus

Protein Variants

Protein Variants Comment Organism
additional information modification of the gene sequence to facilitate protein expression and increase protein solubility, removal of the N-terminal membrane binding motif to enhance protein solubility Astragalus membranaceus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane transmembrane enzyme Astragalus membranaceus 16020
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Organism

Organism UniProt Comment Textmining
Astragalus membranaceus W5S082 var. mongolicus
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Purification (Commentary)

Purification (Comment) Organism
recombinant truncated enzyme, lacking the N-terminal membrane binding motif, from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Astragalus membranaceus

Source Tissue

Source Tissue Comment Organism Textmining
additional information AmI2'H is expressed throughout the entire plant Astragalus membranaceus
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Subunits

Subunits Comment Organism
More secondary structure analysis of AmI2'H Astragalus membranaceus

Synonyms

Synonyms Comment Organism
AmI2'H
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 Astragalus membranaceus
CYP81E42
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 Astragalus membranaceus

Cofactor

Cofactor Comment Organism Structure
cytochrome P-450
-
 Astragalus membranaceus
NADPH-hemoprotein reductase A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._ Astragalus membranaceus

General Information

General Information Comment Organism
metabolism the enzyme is involved in isoflavonoid biosynthesis Astragalus membranaceus
physiological function the enzyme promotes the accumulation of medicarpin malonyl glucoside Astragalus membranaceus