Literature summary for 1.14.14.9 extracted from

Tongsook, C.; Sucharitakul, J.; Thotsaporn, K.; Chaiyen, P.
Interactions with the substrate phenolic group are essential for hydroxylation by the oxygenase component of p-hydroxyphenylacetate 3-hydroxylase (2011), J. Biol. Chem., 286, 44491-44502.

Search for more literature for 1.14.14.9

Cloned(Commentary)

Cloned (Comment)	Organism
-	Acinetobacter baumannii

Protein Variants

Protein Variants	Comment	Organism
H120D	mutant can form C4a-hydroperoxy-FMN, a reactive intermediate necessary for hydroxylation, but cannot hydroxylate 4-hydroxyphenylacetate	Acinetobacter baumannii
H120E	mutant can form C4a-hydroperoxy-FMN, a reactive intermediate necessary for hydroxylation, but cannot hydroxylate 4-hydroxyphenylacetate	Acinetobacter baumannii
H120K	catalyzes hydroxylation with efficiency comparable to that of the wild-type enzyme, the hydroxylation rate constant for H120K is 5.7 per s and the product conversion ratio is 75%, compared to values of 16 s-1 and 90% for the wild-type enzyme	Acinetobacter baumannii
H120N	mutant can form C4a-hydroperoxy-FMN, a reactive intermediate necessary for hydroxylation, but cannot hydroxylate 4-hydroxyphenylacetate	Acinetobacter baumannii
H120Q	mutant can form C4a-hydroperoxy-FMN, a reactive intermediate necessary for hydroxylation, but cannot hydroxylate 4-hydroxyphenylacetate	Acinetobacter baumannii
H120R	mutant is able to catalyze hydroxylation	Acinetobacter baumannii
H120Y	mutant can form C4a-hydroperoxy-FMN, a reactive intermediate necessary for hydroxylation, but cannot hydroxylate 4-hydroxyphenylacetate	Acinetobacter baumannii
S146A	product formation is pH-independent and constant at about 70% over a pH range of 6-10	Acinetobacter baumannii
S146C	product formation decreases from about 65% at pH 6.0 to 27% at pH 10.0	Acinetobacter baumannii

Organism

Organism	UniProt	Comment	Textmining
Acinetobacter baumannii	Q6Q272	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3-hydroxyphenylacetate + FMNH2 + O2	-	Acinetobacter baumannii	3,4-hydroxyphenylacetate + FMN + H2O	hydroxylation rate constant is 16 per s and the product conversion ratio is 90%	?

Synonyms

Synonyms	Comment	Organism
HPA 3-hydroxylase	-	Acinetobacter baumannii

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
additional information	-	hydroxylation reaction of wildtype oxygenase component is pH-independent between pH 6 and 10	Acinetobacter baumannii

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)