Crystallization (Comment) | Organism |
---|---|
flavin reductase component HpaC of 4-hydroxyphenylacetate 3-monooxygenase in a ternary complex with FAD and NAD+, mixing of 0.002-0.005 ml of a solution containing 25 mg/mL HpaCTt-FAD, 5 mM NADH, and 1 mM dithiothreitol with an equal volume of a reservoir solution containing 20% w/v PEG 1000, 10% w/v PEG 8000, and 10% v/v glycerol, and then equilibrating the solution against the reservoir solution, 2 days, X-ray diffraction structure determination and analysis at 1.65-3.3 A resolution | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxyphenylacetate + NAD(P)H + H+ + O2 | Thermus thermophilus | - |
3,4-dihydroxyphenylacetate + NAD(P)+ + H2O | - |
? | |
4-hydroxyphenylacetate + NAD(P)H + H+ + O2 | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
3,4-dihydroxyphenylacetate + NAD(P)+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
- |
- |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxyphenylacetate + NAD(P)H + H+ + O2 | - |
Thermus thermophilus | 3,4-dihydroxyphenylacetate + NAD(P)+ + H2O | - |
? | |
4-hydroxyphenylacetate + NAD(P)H + H+ + O2 | FAD and NAD+ are bound in the groove in the extended and folded conformation, respectively, structure, overview | Thermus thermophilus | 3,4-dihydroxyphenylacetate + NAD(P)+ + H2O | - |
? | |
4-hydroxyphenylacetate + NAD(P)H + H+ + O2 | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | 3,4-dihydroxyphenylacetate + NAD(P)+ + H2O | - |
? | |
4-hydroxyphenylacetate + NAD(P)H + H+ + O2 | FAD and NAD+ are bound in the groove in the extended and folded conformation, respectively, structure, overview | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | 3,4-dihydroxyphenylacetate + NAD(P)+ + H2O | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Thermus thermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | FAD is not tightly bound to HpaC, the flavin reductase component of the enzyme, and is bound in the groove in the extended and folded conformation, binding site structure, overview | Thermus thermophilus | |
FMN | can substitute for FAD in the enzyme assay | Thermus thermophilus | |
NAD(P)H | product NAD+ is bound in the groove in the extended and folded conformation, binding site structure, overview | Thermus thermophilus |