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Literature summary for 1.14.14.9 extracted from
- Molecular characterization of 4-hydroxyphenylacetate 3-hydroxylase of Escherichia coli. A two-protein component enzyme (1994), J. Biol. Chem., 269, 22823-22829.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli DH1 | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 18679 | - |
x * 18679, SDS-PAGE, enzyme is needed for activity | Escherichia coli |
| 58781 | - |
2 * 58781, homodimer, encodes the hydroxylase | Escherichia coli |
| 120000 | - |
gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-hydroxyphenylacetate + NADH + O2 | Escherichia coli | inducible chromosomally encoded meta-cleavage pathway of aromatic degradation | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
strain W, ATCC 11105 derived from ATCC 9637 | - |
| no activity in Escherichia coli K-12 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| affinity chromatography, gel filtration | Escherichia coli |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 4-hydroxyphenylacetate + FADH2 + O2 = 3,4-dihydroxyphenylacetate + FAD + H2O | two enzymes suggested to be required for hydroxylase activity, member of new family of two-component aromatic hydroxylases. The hydroxylase, a flavoprotein, is encoded by hpaB and the coupling protein by hpaC gene | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
120000 Da enzyme encoded by hpaB shows low hydroxylase activity which is increased in the presence of FAD and the coupling protein HpaC | Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 20 mM phosphate buffer, pH 8.0, concentrated with polyethylene glycol 20000 | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-hydroxyphenylacetate + NADH + O2 | - |
Escherichia coli | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? | |
| 4-hydroxyphenylacetate + NADH + O2 | inducible chromosomally encoded meta-cleavage pathway of aromatic degradation | Escherichia coli | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? | |
| phenol + ? | - |
Escherichia coli | catechol + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 18679, SDS-PAGE, enzyme is needed for activity | Escherichia coli |
| dimer | 2 * 58781, homodimer, encodes the hydroxylase | Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | as redox chromophore | Escherichia coli | |
| NADH | - |
Escherichia coli | |
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