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Literature summary for 1.14.14.89 extracted from

  • Hinderer, W.; Flentje, U.; Barz, W.
    Microsomal isoflavone 2'- and 3'-hydroxylases from chickpea (Cicer arietinum L.) cell suspensions induced for pterocarpan phytoalexin formation (1987), FEBS Lett., 214, 101-106.
No PubMed abstract available
Search for more literature for 1.14.14.89

Activating Compound

Activating Compound Comment Organism Structure
additional information cells susceptible to Ascochyta rabiei can be elicitor-induced to 500% activity Cicer arietinum
NADH synergistic effect with NADPH Cicer arietinum
NADH 135% of NADPH activity Cicer arietinum

Inhibitors

Inhibitors Comment Organism Structure
cytochrome c 0.01 mM cytochrome c reduces activity to 14%, 0.1 mM cytochrome c reduces activity to 4% Cicer arietinum
additional information no inhibition by 5 mM KCN and 1 mM EDTA Cicer arietinum

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Cicer arietinum
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
formononetin + [reduced NADPH-hemoprotein reductase] + O2 Cicer arietinum involved in the biosynthesis of the pterocarpin phytoalexin medicarpin 2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ir
formononetin + [reduced NADPH-hemoprotein reductase] + O2 Cicer arietinum cytochrome P450-dependent monooxygenase 2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ir

Organism

Organism UniProt Comment Textmining
Cicer arietinum
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cell culture suspension Cicer arietinum
-
cell suspension culture
-
 Cicer arietinum
-

Storage Stability

Storage Stability Organism
-20°C, 100 mM potassium phosphate, pH 7.5 containing 400 mM sucrose and 3.5 mM 2-mercaptoethanol, 24 h Cicer arietinum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
biochanin A + [reduced NADPH-hemoprotein reductase] + O2
-
 Cicer arietinum 2'-hydroxybiochanin A + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
biochanin A + [reduced NADPH-hemoprotein reductase] + O2
-
 Cicer arietinum 2'-hydroxybiochanin A + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ir
daidzein + [reduced NADPH-hemoprotein reductase] + O2 2.0% of the activity with biochanin A Cicer arietinum 2'-hydroxydaidzein + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
formononetin + [reduced NADPH-hemoprotein reductase] + O2
-
 Cicer arietinum 2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ir
formononetin + [reduced NADPH-hemoprotein reductase] + O2 involved in the biosynthesis of the pterocarpin phytoalexin medicarpin Cicer arietinum 2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ir
formononetin + [reduced NADPH-hemoprotein reductase] + O2 cytochrome P450-dependent monooxygenase Cicer arietinum 2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ir
formononetin + [reduced NADPH-hemoprotein reductase] + O2 78.1% of the activity with biochanin A Cicer arietinum 2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
genistein + [reduced NADPH-hemoprotein reductase] + O2 2.2% of the activity with biochanin A Cicer arietinum 2'-hydroxygenistein + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
additional information monohydroxylation of 4'-methoxyisoflavones, no substrates are genistein and daidzein Cicer arietinum ?
-
 ?

Synonyms

Synonyms Comment Organism
isoflavone 2'-hydroxylase ambiguous Cicer arietinum
isoflavone 2'-monooxygenase ambiguous Cicer arietinum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay conditions Cicer arietinum

Cofactor

Cofactor Comment Organism Structure
additional information no cofactors are FAD and FMN Cicer arietinum