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Literature summary for 1.14.14.84 extracted from

  • Ropp, J.; Gunsalus, I.; Sligar, S.
    Cloning and expression of a member of a new cytochrome P-450 family: Cytochrome P-450lin (CYP111) from Pseudomonas incognita (1993), J. Bacteriol., 175, 6028-6037.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
gene linC, DNA and amino acid sequence determination and analysis, constitutive functional expression in Escherichia coli from plasmid pUC18 under control of the lac promoter Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ heme-containing cytochrome P450 enzyme Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 Pseudomonas putida
-
 (6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O
-
 ?
linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 Pseudomonas putida PpG777
-
 (6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida Q59723 a D110R strain, gene linC
-
Pseudomonas putida PpG777 Q59723 a D110R strain, gene linC
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli by anion exchange chromatography, ammonium sulfate fractionation, gel filtration, and a second step of anion exchange chromatography Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
 Pseudomonas putida (6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O
-
 ?
linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
 Pseudomonas putida PpG777 (6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O
-
 ?

Synonyms

Synonyms Comment Organism
P45O1in
-
 Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
cytochrome P450
-
 Pseudomonas putida
heme
-
 Pseudomonas putida

General Information

General Information Comment Organism
evolution P45O1in is a member of the CYPIll P-450 monooxygenase gene family Pseudomonas putida
metabolism P-4501in catalyzes the 8-methyl hydroxylation of linalool as the first committed step of its utilization by Pseudomonas putida as the sole carbon source Pseudomonas putida
additional information P-4501in exhibits conservation in the axial cysteine heme ligand-containing peptide and the threonine region postulated to form an 02-binding pocket Pseudomonas putida