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Literature summary for 1.14.14.83 extracted from

  • Madyastha, K.M.; Meehan, T.D.; Coscia, C.J.
    Characterization of a cytochrome P-450 dependent monoterpene hydroxylase from the higher plant Vinca rosea (1976), Biochemistry, 15, 1097-1102 .
    View publication on PubMed
Search for more literature for 1.14.14.83

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol 3fold increase in activity in presence of dithiothreitol Catharanthus roseus

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate 0.1 mM, 17% residual activity Catharanthus roseus
CO inhibition increases with increasing CO concentrations and is almost completely reversed with the aid of a light spurce between 420 and 450 nm Catharanthus roseus
iodoacetamide 10 mM, 87% residual activity Catharanthus roseus
Metyrapone 0.1 mM, 41% residual activity Catharanthus roseus
N-ethylmaleimide 1 mM, 58% residual activity Catharanthus roseus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0055
-
 geraniol pH 7.2, 25°C Catharanthus roseus
0.011
-
 nerol pH 7.2, 25°C Catharanthus roseus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Catharanthus roseus 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geraniol + [reduced NADPH-hemoprotein reductase] + O2 Catharanthus roseus
-
 (6E)-8-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
nerol + [reduced NADPH-hemoprotein reductase] + O2 Catharanthus roseus
-
 (6E)-8-hydroxynerol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Catharanthus roseus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
solubilization by treatment with sodium cholate, Renex-30, or Lubrol-WX Catharanthus roseus

Source Tissue

Source Tissue Comment Organism Textmining
seedling etiolated seedling Catharanthus roseus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geraniol + [reduced NADPH-hemoprotein reductase] + O2
-
 Catharanthus roseus (6E)-8-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
nerol + [reduced NADPH-hemoprotein reductase] + O2
-
 Catharanthus roseus (6E)-8-hydroxynerol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Cofactor

Cofactor Comment Organism Structure
cytochrome P450
-
 Catharanthus roseus