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Literature summary for 1.14.14.83 extracted from

  • Sintupachee, S.; Promden, W.; Ngamrojanavanich, N.; Sitthithaworn, W.; De-Eknamkul, W.
    Functional expression of a putative geraniol 8-hydroxylase by reconstitution of bacterially expressed plant CYP76F45 and NADPH-cytochrome P450 reductase CPR I from Croton stellatopilosus Ohba (2015), Phytochemistry, 118, 204-215 .
    View publication on PubMed
Search for more literature for 1.14.14.83

Cloned(Commentary)

Cloned (Comment) Organism
gene CsCPR I, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis and tree, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3). Only simultaneous incubation of the membrane-bound enzymes CsCYP76F45 and CsCPR I with the substrate geraniol and the coenzyme NADPH allows 8-hydroxygeraniol formation Croton stellatopilosus
gene CYP76F45, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis and tree, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3). Only simultaneous incubation of the membrane-bound enzymes CsCYP76F45 and CsCPR I with the substrate geraniol and the coenzyme NADPH allows 8-hydroxygeraniol formation Croton stellatopilosus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane membrane-bound Croton stellatopilosus 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geraniol + [reduced NADPH-hemoprotein reductase] + O2 Croton stellatopilosus
-
 (6E)-8-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
geranylgeraniol + [reduced NADPH-hemoprotein reductase] + O2 Croton stellatopilosus
-
 18-hydroxygeranylgeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
geranylgeraniol + [reduced NADPH-hemoprotein reductase] + O2 Croton stellatopilosus Ohba
-
 18-hydroxygeranylgeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Croton stellatopilosus A0A0A7DLM4
-
-
Croton stellatopilosus A0A0A8KYK4 cf. EC 1.6.2.4
-
Croton stellatopilosus Ohba A0A0A7DLM4
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Croton stellatopilosus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
farnesol + [reduced NADPH-hemoprotein reductase] + O2
-
 Croton stellatopilosus 12-hydroxyfarnesol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
farnesol + [reduced NADPH-hemoprotein reductase] + O2
-
 Croton stellatopilosus Ohba 12-hydroxyfarnesol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
geraniol + [reduced NADPH-hemoprotein reductase] + O2
-
 Croton stellatopilosus (6E)-8-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
geraniol + [reduced NADPH-hemoprotein reductase] + O2
-
 Croton stellatopilosus Ohba (6E)-8-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
geranylgeraniol + [reduced NADPH-hemoprotein reductase] + O2
-
 Croton stellatopilosus 18-hydroxygeranylgeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
geranylgeraniol + [reduced NADPH-hemoprotein reductase] + O2
-
 Croton stellatopilosus Ohba 18-hydroxygeranylgeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
geranylgeraniol + [reduced NADPH-hemoprotein reductase] + O2
-
 Croton stellatopilosus 16-hydroxygeranylgeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
geranylgeraniol + [reduced NADPH-hemoprotein reductase] + O2
-
 Croton stellatopilosus Ohba 16-hydroxygeranylgeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 56700, about, sequence calculation Croton stellatopilosus
? x * 56700, calculated, Cyp76F45 Croton stellatopilosus
? x * 78700, about, sequence calculation, x * 96000, recombinant His-tagged enzyme, SDS-PAGE Croton stellatopilosus

Synonyms

Synonyms Comment Organism
CPR I
-
 Croton stellatopilosus
CYP76F45
-
 Croton stellatopilosus
G8H
-
 Croton stellatopilosus

Cofactor

Cofactor Comment Organism Structure
cytochrome P450 a cytochrome P450 monooxygenase Croton stellatopilosus
cytochrome P450 an NADPH-cytochrome P450 reductase Croton stellatopilosus
FAD
-
 Croton stellatopilosus
FMN
-
 Croton stellatopilosus
heme
-
 Croton stellatopilosus
NADPH an NADPH-cytochrome P450 reductase Croton stellatopilosus

pI Value

Organism Comment pI Value Maximum pI Value
Croton stellatopilosus sequence calculation
-
 5.27
Croton stellatopilosus calculated, Cyp76F45
-
 8.6
Croton stellatopilosus sequence calculation
-
 8.63

General Information

General Information Comment Organism
evolution enzyme CsCPR I belongs to Class I of dicotyledonous CPRs Croton stellatopilosus
evolution enzyme CYP76F45 belongs to the CYP76 family of dicotyledonous CPR class I Croton stellatopilosus
additional information the enzyme contains specific oxygen- and heme-binding sites Croton stellatopilosus
additional information the enzyme contains two FMN-, FAD-, and NADPH-binding sites and one P450-binding site Croton stellatopilosus
physiological function enzymes CYP97C27 and CPR I function as the enzyme geraniol-8-hydroxylase (G8H), which is likely to be involved in the biosynthesis of the indole alkaloid 19-E-vallesamine in Croton stellatopilosus Croton stellatopilosus