Cloned (Comment) | Organism |
---|---|
co-expression of G10H with the NADPH-cytochrome P450 reductase from Arabidopsis thaliana in Spodoptera frugiperda Sf9 cell microsomes using the baculovirus transfection method, quantitative expression analysis | Catharanthus roseus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0158 | - |
geraniol | pH 8.0, 30°C, recombinant enzyme | Catharanthus roseus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Catharanthus roseus | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geraniol + [reduced NADPH-hemoprotein reductase] + O2 | Catharanthus roseus | - |
10-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Catharanthus roseus | Q8VWZ7 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Catharanthus roseus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geraniol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Catharanthus roseus | 10-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
additional information | CrG10H also catalyzed 3'-hydroxylation of the flavanone naringenin to form eriodictyol, a reaction of the flavonoid 3'-hydroxylase. Analysis of the enzyme reactions of the recombinant enzyme with naringenin, apigenin, kaempferol, pcoumaric acid, and ferulic acid, mass spectrometric product analysis, overview | Catharanthus roseus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
G10H | - |
Catharanthus roseus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Catharanthus roseus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.131 | - |
geraniol | pH 8.0, 30°C, recombinant enzyme | Catharanthus roseus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Catharanthus roseus |
General Information | Comment | Organism |
---|---|---|
metabolism | G10H catalyzes the first committed step of the biosynthetic pathway of strictosidine and other terpenoid indole alkaloids, overview | Catharanthus roseus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000008 | - |
geraniol | pH 8.0, 30°C, recombinant enzyme | Catharanthus roseus |