Cloned (Comment) | Organism |
---|---|
cloning from a lambda-ZAPII library, recombinant expression in Saccharomyces cerevisiae strain YPH500 or in enzyme-deficient Catharathus roseus cell line MP183L, complementation | Catharanthus roseus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Catharanthus roseus | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56300 | - |
x * 56300, SDS-PAGE | Catharanthus roseus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geraniol + [reduced NADPH-hemoprotein reductase] + O2 | Catharanthus roseus | - |
10-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Catharanthus roseus | - |
gene CYP76B6 | - |
Purification (Comment) | Organism |
---|---|
native enzyme about 12fold from membranes by anion exchange and omega-amino octyl agarose chromatography, followed by hydophobic interaction chromatography to homogeneity | Catharanthus roseus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geraniol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Catharanthus roseus | 10-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 56300, SDS-PAGE | Catharanthus roseus |
Synonyms | Comment | Organism |
---|---|---|
CYP76B6 | - |
Catharanthus roseus |
G10H | - |
Catharanthus roseus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | - |
Catharanthus roseus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Catharanthus roseus | isoelectric focusing | - |
8.7 |
Organism | Comment | Expression |
---|---|---|
Catharanthus roseus | the stress hormone methyljasmonate strongly induces G10h gene expression coordinately with other terpenoid indole alkaloid biosynthesis genes in Catharanthus roseus cell culture | up |
General Information | Comment | Organism |
---|---|---|
physiological function | geraniol 10-hydroxylase is a cytochrome P450 monooxygenase involved in the biosynthesis of iridoid monoterpenoids and several classes of monoterpenoid alkaloids found in a diverse range of plant species. G10H plays a key regulatory role in terpenoid indole alkaloid biosynthesis | Catharanthus roseus |