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Literature summary for 1.14.14.82 extracted from

  • Schwinn, K.; Miosic, S.; Davies, K.; Thill, J.; Gotame, T.P.; Stich, K.; Halbwirth, H.
    The B-ring hydroxylation pattern of anthocyanins can be determined through activity of the flavonoid 3-hydroxylase on leucoanthocyanidins (2014), Planta, 240, 1003-1010 .
    View publication on PubMed
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 kaempferol pH 7.0, 25°C Fragaria x ananassa
0.001
-
 naringenin pH 7.0, 25°C Fragaria x ananassa
0.0012
-
 naringenin pH 7.3, 25°C Arabidopsis thaliana
0.0013
-
 kaempferol pH 7.0, 25°C Tagetes erecta
0.0019
-
 kaempferol pH 7.3, 25°C Arabidopsis thaliana
0.002
-
 dihydrokaempferol pH 7.0, 25°C Tagetes erecta
0.003
-
 naringenin pH 7.0, 25°C Tagetes erecta
0.0054
-
 apigenin pH 7.0, 25°C Tagetes erecta
0.0058
-
 apigenin pH 7.0, 25°C Fragaria x ananassa
0.0064
-
 apigenin pH 7.3, 25°C Arabidopsis thaliana
0.0074
-
 5-deoxyleucopelargonidin pH 7.3, 25°C Arabidopsis thaliana
0.0079
-
 5-deoxyleucopelargonidin pH 7.0, 25°C Tagetes erecta
0.009
-
 dihydrokaempferol pH 7.0, 25°C Fragaria x ananassa
0.009
-
 dihydrokaempferol pH 7.3, 25°C Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9SD85
-
-
Fragaria x ananassa
-
-
-
Tagetes erecta C1J0N9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-deoxyleucopelargonidin + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana 5-deoxyleucocyanidin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
5-deoxyleucopelargonidin + [reduced NADPH-hemoprotein reductase] + O2
-
 Tagetes erecta 5-deoxyleucocyanidin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
apigenin + [reduced NADPH-hemoprotein reductase] + O2
-
 Fragaria x ananassa luteolin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
apigenin + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana luteolin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
apigenin + [reduced NADPH-hemoprotein reductase] + O2
-
 Tagetes erecta luteolin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
dihydrokaempferol + [reduced NADPH-hemoprotein reductase] + O2
-
 Fragaria x ananassa dihydroquercetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
dihydrokaempferol + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana dihydroquercetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
dihydrokaempferol + [reduced NADPH-hemoprotein reductase] + O2
-
 Tagetes erecta dihydroquercetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
kaempferol + [reduced NADPH-hemoprotein reductase] + O2
-
 Fragaria x ananassa quercetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
kaempferol + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana quercetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
kaempferol + [reduced NADPH-hemoprotein reductase] + O2
-
 Tagetes erecta quercetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
leucopelargonidin + [reduced NADPH-hemoprotein reductase] + O2
-
 Fragaria x ananassa leucocyanidin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
leucopelargonidin + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana leucocyanidin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
leucopelargonidin + [reduced NADPH-hemoprotein reductase] + O2
-
 Tagetes erecta leucocyanidin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
naringenin + [reduced NADPH-hemoprotein reductase] + O2
-
 Fragaria x ananassa eriodictyol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
naringenin + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana eriodictyol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
naringenin + [reduced NADPH-hemoprotein reductase] + O2
-
 Tagetes erecta eriodictyol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1
-
 naringenin pH 7.3, 25°C Arabidopsis thaliana
5.8
-
 kaempferol pH 7.3, 25°C Arabidopsis thaliana
9.3
-
 apigenin pH 7.3, 25°C Arabidopsis thaliana
10.2
-
 kaempferol pH 7.0, 25°C Tagetes erecta
10.6
-
 naringenin pH 7.0, 25°C Fragaria x ananassa
12.3
-
 5-deoxyleucopelargonidin pH 7.3, 25°C Arabidopsis thaliana
12.5
-
 dihydrokaempferol pH 7.0, 25°C Tagetes erecta
12.7
-
 dihydrokaempferol pH 7.3, 25°C Arabidopsis thaliana
13.4
-
 apigenin pH 7.0, 25°C Fragaria x ananassa
17
-
 kaempferol pH 7.0, 25°C Fragaria x ananassa
17
-
 naringenin pH 7.0, 25°C Tagetes erecta
22.1
-
 apigenin pH 7.0, 25°C Tagetes erecta
38.7
-
 5-deoxyleucopelargonidin pH 7.0, 25°C Tagetes erecta
81.4
-
 dihydrokaempferol pH 7.0, 25°C Fragaria x ananassa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
-
 Fragaria x ananassa
7.3
-
-
 Arabidopsis thaliana
7.3
-
-
 Tagetes erecta

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
900
-
 dihydrokaempferol pH 7.0, 25°C Fragaria x ananassa
1400
-
 dihydrokaempferol pH 7.3, 25°C Arabidopsis thaliana
1500
-
 apigenin pH 7.3, 25°C Arabidopsis thaliana
1700
-
 kaempferol pH 7.0, 25°C Fragaria x ananassa
1700
-
 5-deoxyleucopelargonidin pH 7.3, 25°C Arabidopsis thaliana
1800
-
 naringenin pH 7.3, 25°C Arabidopsis thaliana
2300
-
 apigenin pH 7.0, 25°C Fragaria x ananassa
3100
-
 kaempferol pH 7.3, 25°C Arabidopsis thaliana
4100
-
 apigenin pH 7.0, 25°C Tagetes erecta
5000
-
 5-deoxyleucopelargonidin pH 7.0, 25°C Tagetes erecta
5600
-
 naringenin pH 7.0, 25°C Tagetes erecta
6300
-
 dihydrokaempferol pH 7.0, 25°C Tagetes erecta
7700
-
 kaempferol pH 7.0, 25°C Tagetes erecta
10000
-
 naringenin pH 7.0, 25°C Fragaria x ananassa