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Literature summary for 1.14.14.81 extracted from

  • Nakatsuka, T.; Mishiba, K.I.; Kubota, A.; Abe, Y.; Yamamura, S.; Nakamura, N.; Tanaka, Y.; Nishihara, M.
    Genetic engineering of novel flower colour by suppression of anthocyanin modification genes in gentian (2009), J. Plant Physiol., 167, 231-237.
    View publication on PubMed
Search for more literature for 1.14.14.81

Cloned(Commentary)

Cloned (Comment) Organism
PCR-amplification, transformation into Agrobacterium tumefaciens EHA101 by electroporation used to transform the blue-flowered Gentiana triflora x Gentiana scabra cultivar Albireo via leaf disc infection Gentiana triflora

Protein Variants

Protein Variants Comment Organism
additional information introduction of a binary vector to silence flavonoid 3',5'-hydroxylase and anthocyanin 5,3'-aromatic acyltransferase via RNAi silencing downregulating gentiodelphin biosynthesis, thus affecting flower color Gentiana triflora

Inhibitors

Inhibitors Comment Organism Structure
RNAi reduces transcript levels, thus affecting flower color, acylated delphinidins (dominating in the wild-type) including gentiodelphin are decreased to 12.0% while non-acylated delphinidins are increased to 66.0 and 69.7% (15.2% in wild-type) of total anthocyanins in two transformed strains (clone 1 lilac, clone 15 pale blue) Gentiana triflora

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Gentiana triflora delphinidin biosynthesis ?
-
 ?

Organism

Organism UniProt Comment Textmining
Gentiana triflora Q96581 Gentiana triflora x Gentiana scabra cultivar Albireo, ornamental gentian plant
-

Source Tissue

Source Tissue Comment Organism Textmining
petal flower developmental stage 3 Gentiana triflora
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information delphinidin biosynthesis Gentiana triflora ?
-
 ?

Synonyms

Synonyms Comment Organism
F3'5'H
-
 Gentiana triflora
flavonoid 3',5'-hydroxylase
-
 Gentiana triflora

Expression

Organism Comment Expression
Gentiana triflora chimeric RNAi technology silences transcription of enzyme, in clone 15 (pale blue) more than in clone 1 (lilac) down

General Information

General Information Comment Organism
metabolism essential in gentiodelphin (delphinidin 3-O-glucosyl-5-O-caffeoyl-glucosyl-3'-O-caffeoyl-glucoside) biosynthesis via flavonoid production causing the flower color of petals Gentiana triflora