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Literature summary for 1.14.14.81 extracted from

  • Seitz, C.; Vitten, M.; Steinbach, P.; Hartl, S.; Hirsche, J.; Rathje, W.; Treutter, D.; Forkmann, G.
    Redirection of anthocyanin synthesis in Osteospermum hybrida by a two-enzyme manipulation strategy (2007), Phytochemistry, 68, 824-833.
    View publication on PubMed
Search for more literature for 1.14.14.81

Protein Variants

Protein Variants Comment Organism
additional information redirection of anthocyanin synthesis in Osteospermum hybrida towards pelargonidin derivatives, which are not synthesized in wild-type plants, by expression of Gerbera hybrida dihydroflavonol 4-reductase, the mutant plants show altered flower colours due to synthesis of pelargonidin anthocyaninsssing Gerbera DFR with a construct or RNAi-mediated suppression of F3'5'H activity results in double transgenic plants accumulating predominantly pelargonidin derivatives in flowers, overview Osteospermum hybrid cultivar

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dihydrokaempferol + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 Osteospermum hybrid cultivar
-
 dihydromyricetin + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
additional information Osteospermum hybrid cultivar the enzyme is involved in biosynthesis of delphinidin-derived anthocyanins which are responsible for the rose to lilac flower colours of the petals, overview ?
-
 ?
naringenin + [reduced NADPH-hemoprotein reductase] + O2 Osteospermum hybrid cultivar
-
 (2S)-eriodictyol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Osteospermum hybrid cultivar Q304Q4 cv. Kalanga rosy
-

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
 Osteospermum hybrid cultivar
-
petal
-
 Osteospermum hybrid cultivar
-
ray floret F3'5'H activity in ray floret enzyme extracts of 3 developmental stages, overview Osteospermum hybrid cultivar
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 F3'5'H activity in ray floret enzyme extracts of 3 developmental stages, overview Osteospermum hybrid cultivar

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydrokaempferol + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
 Osteospermum hybrid cultivar dihydromyricetin + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
additional information the enzyme is involved in biosynthesis of delphinidin-derived anthocyanins which are responsible for the rose to lilac flower colours of the petals, overview Osteospermum hybrid cultivar ?
-
 ?
naringenin + [reduced NADPH-hemoprotein reductase] + O2
-
 Osteospermum hybrid cultivar (2S)-eriodictyol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

Synonyms Comment Organism
F3'5'H
-
 Osteospermum hybrid cultivar
flavonoid 3',5'-hydroxylase
-
 Osteospermum hybrid cultivar

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Osteospermum hybrid cultivar

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Osteospermum hybrid cultivar