Cloned (Comment) | Organism |
---|---|
coexpressed with yeast NADPH-cytochrome P450 reductase in Saccharomyces cerevisiae | Chamberlinius hualienensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0066 | - |
2-methyl phenylacetonitrile | pH 6.0, 20°C | Chamberlinius hualienensis | |
0.0069 | - |
3-methyl phenylacetonitrile | pH 6.0, 20°C | Chamberlinius hualienensis | |
0.0092 | - |
phenylacetonitrile | pH 6.0, 20°C | Chamberlinius hualienensis | |
0.0158 | - |
4-Hydroxyphenylacetonitrile | pH 6.0, 20°C | Chamberlinius hualienensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methyl phenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2 | Chamberlinius hualienensis | - |
(2R)-hydroxy-(2-methylphenyl)acetonitrile + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
3-methyl phenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2 | Chamberlinius hualienensis | - |
(2R)-hydroxy-(3-methylphenyl)acetonitrile + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
4-hydroxyphenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2 | Chamberlinius hualienensis | - |
(2R)-hydroxy-(4-hydroxyphenyl)acetonitrile + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
additional information | Chamberlinius hualienensis | no substrate: (E/Z)-phenylacetaldoxime | ? | - |
? | |
additional information | Chamberlinius hualienensis | no substrates: 3-phenylpropionitrile, indole-3-acetonitrile, 3-phenyl-1-propyne or 3-phenyl-1-propene | ? | - |
? | |
phenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2 | Chamberlinius hualienensis | - |
(R)-mandelonitrile + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chamberlinius hualienensis | A0A1J1DVP9 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methyl phenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2 | - |
Chamberlinius hualienensis | (2R)-hydroxy-(2-methylphenyl)acetonitrile + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
3-methyl phenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2 | - |
Chamberlinius hualienensis | (2R)-hydroxy-(3-methylphenyl)acetonitrile + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
4-hydroxyphenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2 | - |
Chamberlinius hualienensis | (2R)-hydroxy-(4-hydroxyphenyl)acetonitrile + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
additional information | no substrate: (E/Z)-phenylacetaldoxime | Chamberlinius hualienensis | ? | - |
? | |
additional information | no substrates: 3-phenylpropionitrile, indole-3-acetonitrile, 3-phenyl-1-propyne or 3-phenyl-1-propene | Chamberlinius hualienensis | ? | - |
? | |
phenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2 | - |
Chamberlinius hualienensis | (R)-mandelonitrile + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP3201B1 | - |
Chamberlinius hualienensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Chamberlinius hualienensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
stable for 20 min | Chamberlinius hualienensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.71 | - |
4-Hydroxyphenylacetonitrile | pH 6.0, 20°C | Chamberlinius hualienensis | |
1.09 | - |
3-methyl phenylacetonitrile | pH 6.0, 20°C | Chamberlinius hualienensis | |
1.12 | - |
2-methyl phenylacetonitrile | pH 6.0, 20°C | Chamberlinius hualienensis | |
3.07 | - |
phenylacetonitrile | pH 6.0, 20°C | Chamberlinius hualienensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Chamberlinius hualienensis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | - |
Chamberlinius hualienensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | - |
Chamberlinius hualienensis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
45 | - |
4-Hydroxyphenylacetonitrile | pH 6.0, 20°C | Chamberlinius hualienensis | |
158 | - |
3-methyl phenylacetonitrile | pH 6.0, 20°C | Chamberlinius hualienensis | |
170 | - |
2-methyl phenylacetonitrile | pH 6.0, 20°C | Chamberlinius hualienensis | |
335 | - |
phenylacetonitrile | pH 6.0, 20°C | Chamberlinius hualienensis |