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Literature summary for 1.14.14.76 extracted from

  • Wu, Y.; Hillwig, M.L.; Wang, Q.; Peters, R.J.
    Parsing a multifunctional biosynthetic gene cluster from rice: Biochemical characterization of CYP71Z6 & 7 (2011), FEBS Lett., 585, 3446-3451.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli C41 cells Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
 ent-isokaurene pH and temperature not specified in the publication Oryza sativa

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ent-isokaurene + [reduced NADPH-hemoprotein reductase] + O2
-
 Oryza sativa ent-2alpha-hydroxyisokaurene + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ir
additional information the enzyme does not react with ent-cassadiene Oryza sativa ?
-
 ?

Synonyms

Synonyms Comment Organism
CYP71Z6
-
 Oryza sativa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.01
-
 ent-isokaurene pH and temperature not specified in the publication Oryza sativa

General Information

General Information Comment Organism
metabolism the enzyme acts as a C2-hydroxylase involved in a latter step of phytocassane biosynthesis Oryza sativa