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Literature summary for 1.14.14.70 extracted from
- CYP701A8: a rice ent-kaurene oxidase paralog diverted to more specialized diterpenoid metabolism (2012), Plant Physiol., 158, 1418-1425.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli strain C41 | Oryza sativa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
ent-cassadiene | pH and temperature not specified in the publication | Oryza sativa |  |
| 0.0023 | - |
ent-sandaracopimaradiene | pH and temperature not specified in the publication | Oryza sativa | |
| 0.007 | - |
ent-kaurene | pH and temperature not specified in the publication | Oryza sativa | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ent-cassadiene + [reduced NADPH-hemoprotein reductase] + O2 | Oryza sativa | - |
3alpha-hydroxy-ent-cassadiene + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| ent-kaurene + [reduced NADPH-hemoprotein reductase] + O2 | Oryza sativa | - |
3alpha-hydroxy-ent-kaurene + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| ent-sandaracopimaradiene + NADPH + H+ + O2 | Oryza sativa | - |
ent-sandaracopimaradien-3beta-ol + NADP+ + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryza sativa | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ent-cassadiene + [reduced NADPH-hemoprotein reductase] + O2 | - |
Oryza sativa | 3alpha-hydroxy-ent-cassadiene + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| ent-kaurene + [reduced NADPH-hemoprotein reductase] + O2 | - |
Oryza sativa | 3alpha-hydroxy-ent-kaurene + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| ent-sandaracopimaradiene + NADPH + H+ + O2 | - |
Oryza sativa | ent-sandaracopimaradien-3beta-ol + NADP+ + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CYP701A8 | - |
Oryza sativa |
| KOL4 | - |
Oryza sativa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.013 | - |
ent-sandaracopimaradiene | pH and temperature not specified in the publication | Oryza sativa | |
| 0.017 | - |
ent-cassadiene | pH and temperature not specified in the publication | Oryza sativa | |
| 0.022 | - |
ent-kaurene | pH and temperature not specified in the publication | Oryza sativa | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Oryza sativa | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3 | - |
ent-kaurene | pH and temperature not specified in the publication | Oryza sativa | |
| 6 | - |
ent-sandaracopimaradiene | pH and temperature not specified in the publication | Oryza sativa | |
| 9 | - |
ent-cassadiene | pH and temperature not specified in the publication | Oryza sativa | |
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Release 2023.1 (January 2023)
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