BRENDA - Enzyme Database
show all sequences of 1.14.14.61

Elucidation of the biosynthesis of carnosic acid and its reconstitution in yeast

Scheler, U.; Brandt, W.; Porzel, A.; Rothe, K.; Manzano, D.; Bozic, D.; Papaefthimiou, D.; Balcke, G.; Henning, A.; Lohse, S.; Marillonnet, S.; Kanellis, A.; Ferrer, A.; Tissier, A.; Nat. Commun. 7, 12942 (2016)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Nicotiana benthamiana leaf
Salvia rosmarinus
expression in Nicotiana benthamiana leaf
Salvia fruticosa
Organism
Organism
UniProt
Commentary
Textmining
Salvia fruticosa
-
-
-
Salvia pomifera
A0A0S1TPC7
-
-
Salvia rosmarinus
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
trichome
glandular trichome
Salvia rosmarinus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
11-hydroxyferruginol + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
745860
Salvia rosmarinus
carnosic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
11-hydroxyferruginol + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
745860
Salvia pomifera
carnosic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
11-hydroxyferruginol + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
745860
Salvia fruticosa
carnosic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
additional information
reaction intermediates are 11,20-dohydroxyferruginol and carnosaldehyde, which itself spontaneously oxidizes to carnosol. The enzyme can also oxidize miltiradiene and ferruginol, the latter leading to pisifieric acid, albeit with much lower efficiency, indicating 11-hydroxyferruginol is their preferred substrate
745860
Salvia rosmarinus
?
-
-
-
?
additional information
reaction intermediates are 11,20-dohydroxyferruginol and carnosaldehyde, which itself spontaneously oxidizes to carnosol. The enzyme can also oxidize miltiradiene and ferruginol, the latter leading to pisifieric acid, albeit with much lower efficiency, indicating 11-hydroxyferruginol is their preferred substrate
745860
Salvia pomifera
?
-
-
-
?
additional information
reaction intermediates are 11,20-dohydroxyferruginol and carnosaldehyde, which itself spontaneously oxidizes to carnosol. The enzyme can also oxidize miltiradiene and ferruginol, thelatter leading to pisifieric acid, albeit with much lower efficiency, indicating 11-hydroxyferruginol is their preferred substrate
745860
Salvia fruticosa
?
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
CYP76AK6
-
Salvia pomifera
CYP76AK7
-
Salvia fruticosa
CYP76AK8
-
Salvia rosmarinus
CYP76AK8
-
Salvia fruticosa
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Nicotiana benthamiana leaf
Salvia rosmarinus
expression in Nicotiana benthamiana leaf
Salvia fruticosa
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
trichome
glandular trichome
Salvia rosmarinus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
11-hydroxyferruginol + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
745860
Salvia rosmarinus
carnosic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
11-hydroxyferruginol + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
745860
Salvia pomifera
carnosic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
11-hydroxyferruginol + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
745860
Salvia fruticosa
carnosic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
additional information
reaction intermediates are 11,20-dohydroxyferruginol and carnosaldehyde, which itself spontaneously oxidizes to carnosol. The enzyme can also oxidize miltiradiene and ferruginol, the latter leading to pisifieric acid, albeit with much lower efficiency, indicating 11-hydroxyferruginol is their preferred substrate
745860
Salvia rosmarinus
?
-
-
-
?
additional information
reaction intermediates are 11,20-dohydroxyferruginol and carnosaldehyde, which itself spontaneously oxidizes to carnosol. The enzyme can also oxidize miltiradiene and ferruginol, the latter leading to pisifieric acid, albeit with much lower efficiency, indicating 11-hydroxyferruginol is their preferred substrate
745860
Salvia pomifera
?
-
-
-
?
additional information
reaction intermediates are 11,20-dohydroxyferruginol and carnosaldehyde, which itself spontaneously oxidizes to carnosol. The enzyme can also oxidize miltiradiene and ferruginol, thelatter leading to pisifieric acid, albeit with much lower efficiency, indicating 11-hydroxyferruginol is their preferred substrate
745860
Salvia fruticosa
?
-
-
-
?
Other publictions for EC 1.14.14.61
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745860
Scheler
Elucidation of the biosynthes ...
Salvia fruticosa, Salvia rosmarinus, Salvia pomifera
Nat. Commun.
7
12942
2016
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-
2
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3
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1
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6
-
4
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2
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1
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6
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-
746312
Ignea
Carnosic acid biosynthesis el ...
Salvia rosmarinus, Salvia pomifera
Proc. Natl. Acad. Sci. USA
113
3681-3686
2016
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4
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1
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6
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2
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2
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2
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1
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6
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