Literature summary for 1.14.14.60 extracted from

Ignea, C.; Athanasakoglou, A.; Andreadelli, A.; Apostolaki, M.; Iakovides, M.; Stephanou, E.G.; Makris, A.M.; Kampranis, S.C.
Overcoming the plasticity of plant specialized metabolism for selective diterpene production in yeast (2017), Sci. Rep., 7, 8855 .

Search for more literature for 1.14.14.60

Application

Application	Comment	Organism
synthesis	coexpression of mutant F112L or mutant V296L and cytochrome Cyp76AK6 in yeast leads to overall improvements of 24- and 14fold for pisiferic acid and salviol, respectively, compared to wild-type	Salvia pomifera

Cloned(Commentary)

Cloned (Comment)	Organism
expression Saccaromyces cerevisiae	Salvia pomifera

Crystallization (Commentary)

Crystallization (Comment)	Organism
molecular modeling of structure	Salvia pomifera

Protein Variants

Protein Variants	Comment	Organism
F112L	mutant catalyzes ferruginol production in yeast 2 times more efficiently than the wild-type enzyme, while production of 11-hydroxy-ferruginol is abolished. mutant does not oxidize miltiradiene, reaction of EC 1.14.13.190, or manoyl oxide	Salvia pomifera
V296L	mutant produces 1.5 times more ferruginol, but 8fold less 11-hydroxy-ferruginol and 20fold less 11beta-hydroxy-manoyl oxide than the wild-type enzyme. mutant does not oxidize miltiradiene, reaction of EC 1.14.13.190	Salvia pomifera

Organism

Organism	UniProt	Comment	Textmining
Salvia pomifera	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
trichome	glandular trichome	Salvia pomifera	-

Synonyms

Synonyms	Comment	Organism
CYP76AH24	-	Salvia pomifera

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Release 2023.1 (January 2023)