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show all sequences of 1.14.14.56

Oxidation of 1,8-cineole, the monoterpene cyclic ether originated from Eucalyptus polybractea, by cytochrome P450 3A enzymes in rat and human liver microsomes

Miyazawa, M.; Shindo, M.; Shimada, T.; Drug Metab. Dispos. 29, 200-205 (2001)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
(3beta,16alpha)-3-hydroxy-20-oxopregn-5-ene-16-carbonitrile
induces activity
Rattus norvegicus
Phenobarbital
induces activity
Rattus norvegicus
Cloned(Commentary)
Commentary
Organism
expression in insect cells
Rattus norvegicus
Inhibitors
Inhibitors
Commentary
Organism
Structure
ketoconazole
significant inhibition
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
-
1,8-cineole
pH 7.4, 37°C, after treatment of animals with pregenolone-16alpha-carbonitrile
Rattus norvegicus
0.05
-
1,8-cineole
pH 7.4, 37°C
Rattus norvegicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Rattus norvegicus
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,8-cineole + [reduced NADPH-hemoprotein reductase] + H+ + O2
-
717531
Rattus norvegicus
2-exo-hydroxy-1,8-cineole + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
(3beta,16alpha)-3-hydroxy-20-oxopregn-5-ene-16-carbonitrile
induces activity
Rattus norvegicus
Phenobarbital
induces activity
Rattus norvegicus
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in insect cells
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
ketoconazole
significant inhibition
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
-
1,8-cineole
pH 7.4, 37°C, after treatment of animals with pregenolone-16alpha-carbonitrile
Rattus norvegicus
0.05
-
1,8-cineole
pH 7.4, 37°C
Rattus norvegicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Rattus norvegicus
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,8-cineole + [reduced NADPH-hemoprotein reductase] + H+ + O2
-
717531
Rattus norvegicus
2-exo-hydroxy-1,8-cineole + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
General Information
General Information
Commentary
Organism
physiological function
CYP3A4 is a major enzyme involved in the oxidation of 1,8-cineole by human liver microsomes, as there is a good correlation between CYP3A4 contents and 1,8-cineole 2-hydroxylation activities in liver microsomes of eighteen human samples and of various recombinant human P450 enzymes examined, CYP3A4 has the highest activities for 1,8-cineole 2-hydroxylation
Rattus norvegicus
General Information (protein specific)
General Information
Commentary
Organism
physiological function
CYP3A4 is a major enzyme involved in the oxidation of 1,8-cineole by human liver microsomes, as there is a good correlation between CYP3A4 contents and 1,8-cineole 2-hydroxylation activities in liver microsomes of eighteen human samples and of various recombinant human P450 enzymes examined, CYP3A4 has the highest activities for 1,8-cineole 2-hydroxylation
Rattus norvegicus
Other publictions for EC 1.14.14.56
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
717270
Duisken
Metabolism of 1,8-cineole by h ...
Homo sapiens
Biochim. Biophys. Acta
1722
304-311
2005
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1
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4
1
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2
2
717531
Miyazawa
Oxidation of 1,8-cineole, the ...
Rattus norvegicus
Drug Metab. Dispos.
29
200-205
2001
2
-
1
-
-
-
1
2
1
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-
-
-
1
-
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1
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1
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1
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1
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1
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1
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1
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1
1
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-
718453
Miyazawa
Roles of cytochrome P450 3A en ...
Homo sapiens, Rattus norvegicus
Xenobiotica
31
713-723
2001
2
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2
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2
3
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