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Literature summary for 1.14.14.52 extracted from

  • van Beilen, J.B.; Holtackers, R.; Luscher, D.; Bauer, U.; Witholt, B.; Duetz, W.A.
    Biocatalytic production of perillyl alcohol from limonene by using a novel Mycobacterium sp. cytochrome P450 alkane hydroxylase expressed in Pseudomonas putida (2005), Appl. Environ. Microbiol., 71, 1737-1744.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.14.52

Cloned(Commentary)

Cloned (Comment) Organism
expression in n-octane grown Pseudomonas putida Mycobacterium sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Mycobacterium sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 x * 48000, SDS-PAGE Mycobacterium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(-)-(S)-limonene + NADPH + O2 Mycobacterium sp.
-
 (-)-perillyl alcohol + NADP+ + H2O
-
 ?
(-)-(S)-limonene + NADPH + O2 Mycobacterium sp. HXN-1500
-
 (-)-perillyl alcohol + NADP+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium sp.
-
 HXN-1500
-
Mycobacterium sp. HXN-1500
-
 HXN-1500
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, phenyl Sepharose, Source Q15 Mycobacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(-)-(S)-limonene + NADPH + O2
-
 Mycobacterium sp. (-)-perillyl alcohol + NADP+ + H2O
-
 ?
(-)-(S)-limonene + NADPH + O2
-
 Mycobacterium sp. HXN-1500 (-)-perillyl alcohol + NADP+ + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 48000, SDS-PAGE Mycobacterium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
-
 50% loss of activity after 6 h Mycobacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Mycobacterium sp.

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8 almost no activity at pH 5.5 and pH 8.0, respectively Mycobacterium sp.