BRENDA - Enzyme Database
show all sequences of 1.14.14.51

Cytochrome P-450 dependent (+)-limonene-6-hydroxylation in fruits of caraway (Carum carvi)

Bouwmeester, H.; Konings, M.; Gershenzon, J.; Karp, F.; Croteau, R.; Phytochemistry 50, 243-248 (1999)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
clotrimazole
-
Carum carvi
CO
-
Carum carvi
cytochrome c
-
Carum carvi
Metyrapone
-
Carum carvi
miconazole
-
Carum carvi
additional information
blue light strongly decreases enzyme activity
Carum carvi
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Carum carvi
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(S)-limonene + [reduced NADPH-hemoprotein reductase] + O2
Carum carvi
-
(-)-trans-carveol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Carum carvi
-
variant Karzo
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
fruit
-
Carum carvi
-
Storage Stability
Storage Stability
Organism
-80°C, enzyme in microsomal pellet, over 1 year, no loss of activity
Carum carvi
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(S)-limonene + [reduced NADPH-hemoprotein reductase] + O2
-
743432
Carum carvi
(-)-trans-carveol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
(+)-limonene-6-hydroxylase
-
Carum carvi
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.3
8.4
half-maximal activities at pH 6.3 and 8.4
Carum carvi
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
-
Carum carvi
FMN
-
Carum carvi
NADPH
-
Carum carvi
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
-
Carum carvi
FMN
-
Carum carvi
NADPH
-
Carum carvi
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
clotrimazole
-
Carum carvi
CO
-
Carum carvi
cytochrome c
-
Carum carvi
Metyrapone
-
Carum carvi
miconazole
-
Carum carvi
additional information
blue light strongly decreases enzyme activity
Carum carvi
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Carum carvi
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(S)-limonene + [reduced NADPH-hemoprotein reductase] + O2
Carum carvi
-
(-)-trans-carveol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
fruit
-
Carum carvi
-
Storage Stability (protein specific)
Storage Stability
Organism
-80°C, enzyme in microsomal pellet, over 1 year, no loss of activity
Carum carvi
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(S)-limonene + [reduced NADPH-hemoprotein reductase] + O2
-
743432
Carum carvi
(-)-trans-carveol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.3
8.4
half-maximal activities at pH 6.3 and 8.4
Carum carvi
Other publictions for EC 1.14.14.51
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
660251
Turner
Organization of monoterpene bi ...
Mentha spicata
Plant Physiol.
136
4215-4227
2004
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656954
Carter
Monoterpene biosynthesis pathw ...
Mentha spicata
Phytochemistry
64
425-433
2003
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1
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3
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717888
Hamada
Stereoselective biotransformat ...
Synechococcus sp., Synechococcus sp. PCC 7942
J. Biosci. Bioeng.
96
581-584
2003
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19
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8
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1
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1
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390094
Wuest
Hydroxylation of specifically ...
Mentha spicata
Biochemistry
41
1820-1827
2002
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1
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2
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658534
Shimada
Species differences in the met ...
Canis lupus familiaris, Cavia porcellus, Oryctolagus cuniculus, Macaca fascicularis, Homo sapiens, Mus musculus, Rattus norvegicus
Drug Metab. Pharmacokinet.
17
507-515
2002
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-
-
-
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7
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7
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7
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7
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7
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7
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7
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390089
Wust
Hydroxylation of limonene enan ...
Mentha spicata
Arch. Biochem. Biophys.
387
125-136
2001
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2
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390086
Schalk
A single amino acid substituti ...
Mentha spicata
Proc. Natl. Acad. Sci. USA
97
11948-11953
2000
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5
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1
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1
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390088
Haudenschild
Functional expression of regio ...
Mentha spicata
Arch. Biochem. Biophys.
379
127-136
2000
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-
1
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1
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1
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741662
Cheong
Cloning and expression of the ...
Geobacillus stearothermophilus, Geobacillus stearothermophilus BR388
Appl. Biochem. Biotechnol.
84-86
903-915
2000
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1
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2
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2
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7
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15
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1
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1
3
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15
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1
1
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1
1
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390087
Lupien
Regiospecific cytochrome P450 ...
Mentha spicata
Arch. Biochem. Biophys.
368
181-192
1999
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1
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1
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1
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4
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743432
Bouwmeester
-
Cytochrome P-450 dependent (+ ...
Carum carvi
Phytochemistry
50
243-248
1999
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-
6
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1
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1
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1
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1
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1
1
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1
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3
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3
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6
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1
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1
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1
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1
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390097
Lupien
Cytochrome P450 limonene hydro ...
Mentha spicata
Drug Metabol. Drug Interact.
12
245-260
1995
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1
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1
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5
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1
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390085
Gershenzon
Isolation of secretory cells f ...
Mentha spicata
Anal. Biochem.
200
130-138
1992
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3
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1
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1
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2
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1
1
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1
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3
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1
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1
1
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1
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1
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390084
Croteau
Biochemical characterization o ...
Mentha x gracilis
Plant Physiol.
96
744-752
1991
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2
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1
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1
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4
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4
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390083
Karp
Monoterpene biosynthesis: spec ...
Mentha spicata
Arch. Biochem. Biophys.
276
219-226
1990
-
-
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-
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9
1
1
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1
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1
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1
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1
1
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6
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1
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9
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6
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1
1
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286368
Gershenzon
Biochemical and histochemical ...
Mentha spicata
Plant Physiol.
89
1351-1357
1989
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