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Literature summary for 1.14.14.51 extracted from

  • Cheong, T.; Oriel, P.
    Cloning and expression of the limonene hydroxylase of Bacillus stearothermophilus BR388 and utilization in two-phase limonene conversions (2000), Appl. Biochem. Biotechnol., 84-86, 903-915 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Geobacillus stearothermophilus

Localization

Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-limonene + [reduced NADH-hemoprotein reductase] + O2 Geobacillus stearothermophilus
-
(-)-trans-carveol + [oxidized NADH-hemoprotein reductase] + H2O
-
?
(S)-limonene + [reduced NADH-hemoprotein reductase] + O2 Geobacillus stearothermophilus BR388
-
(-)-trans-carveol + [oxidized NADH-hemoprotein reductase] + H2O
-
?

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus O85057
-
-
Geobacillus stearothermophilus BR388 O85057
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-limonene + [reduced NADH-hemoprotein reductase] + O2
-
Geobacillus stearothermophilus (-)-trans-carveol + [oxidized NADH-hemoprotein reductase] + H2O
-
?
(S)-limonene + [reduced NADH-hemoprotein reductase] + O2
-
Geobacillus stearothermophilus BR388 (-)-trans-carveol + [oxidized NADH-hemoprotein reductase] + H2O
-
?
1-cyclohexene 1-methanol + NAD+ 9.6% activity compared to perillyl alcohol Geobacillus stearothermophilus 1-cyclohexen-1-carbaldehyde + NADH + H+
-
?
1-cyclohexene 1-methanol + NAD+ 9.6% activity compared to perillyl alcohol Geobacillus stearothermophilus BR388 1-cyclohexen-1-carbaldehyde + NADH + H+
-
?
4-isopropyl benzyl alcohol + NAD+ 42.2% activity compared to perillyl alcohol Geobacillus stearothermophilus 4-isopropylbenzyl aldehyde + NADH
-
?
4-isopropyl benzyl alcohol + NAD+ 42.2% activity compared to perillyl alcohol Geobacillus stearothermophilus BR388 4-isopropylbenzyl aldehyde + NADH
-
?
4-methyl benzyl alcohol + NAD+ 17.1% activity compared to perillyl alcohol Geobacillus stearothermophilus ? + NADH
-
?
benzyl alcohol + NAD+ 3.1% activity compared to perillyl alcohol Geobacillus stearothermophilus benzaldehyde + NADH
-
?
benzyl alcohol + NAD+ 3.1% activity compared to perillyl alcohol Geobacillus stearothermophilus BR388 benzaldehyde + NADH
-
?
carveol + NAD+ 86% activity compared to perillyl alcohol Geobacillus stearothermophilus ? + NADH
-
?
cyclohexyl methanol + NAD+ 15.8% activity compared to perillyl alcohol Geobacillus stearothermophilus cyclohexylaldehyde + NADH
-
?
myrtenol + NAD+ 4.0% activity compared to perillyl alcohol Geobacillus stearothermophilus myrtenal + NADH
-
?
perillyl alcohol + NAD+ 100% activity Geobacillus stearothermophilus perillyl aldehyde + NADH
-
r
perillyl alcohol + NAD+ 100% activity Geobacillus stearothermophilus BR388 perillyl aldehyde + NADH
-
r
perillyl aldehyde + NADH
-
Geobacillus stearothermophilus perillyl alcohol + NAD+
-
r

Synonyms

Synonyms Comment Organism
limonene hydroxylase
-
Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Geobacillus stearothermophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 55 about 35% activity at 30°C, about 55% activity at 37°C, about 90% activity at 40°C, 100% activity at 45°C, about 75% activity at 50°C, about 30% activity at 55°C Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
-
Geobacillus stearothermophilus

pH Range

pH Minimum pH Maximum Comment Organism
5.5 10 about 40% activity at pH 5.5, about 75% activity at pH 6.5, about 90% activity at pH 7.0, 100% activity at 8.0, about 75% activity at pH 9.0, about 40% activity at pH 10.0 Geobacillus stearothermophilus

Cofactor

Cofactor Comment Organism Structure
FAD stimulates the hydroxylation reaction Geobacillus stearothermophilus
NAD+ stimulates the dehydrogenase reaction Geobacillus stearothermophilus
NADH stimulates the hydroxylation reaction Geobacillus stearothermophilus