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Literature summary for 1.14.14.5 extracted from

  • Zhan, X.; Carpenter, R.A.; Ellis, H.R.
    Catalytic importance of the substrate binding order for the FMNH2-dependent alkanesulfonate monooxygenase enzyme (2008), Biochemistry, 47, 2221-2230.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics of reaction of SsuD with FMNH2, rapid reaction and stopped flow kinetic analyses, dissociation constants for FMN and FMNH2, detailed overview Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli the two-component alkanesulfonate monooxygenase system from Escherichia coli includes an FMN reductase, SsuE, and an FMNH2-dependent alkanesulfonate monooxygenase, SsuD, involved in the acquisition of sulfur from alkanesulfonates during sulfur starvation, overview ?
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?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Reaction

Reaction Comment Organism Reaction ID
an alkanesulfonate + FMNH2 + O2 = an aldehyde + FMN + sulfite + H2O mechanism of flavin reduction in the alkanesulfonate monooxygenase system, overview Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the two-component alkanesulfonate monooxygenase system from Escherichia coli includes an FMN reductase, SsuE, and an FMNH2-dependent alkanesulfonate monooxygenase, SsuD, involved in the acquisition of sulfur from alkanesulfonates during sulfur starvation, overview Escherichia coli ?
-
?
octanesulfonate + FMNH2 + O2 SsuD shows a clear preference for FMNH2, reaction via C4a-(hydro)peroxyflavin intermediate Escherichia coli octanal + FMN + sulfite + H2O
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?

Synonyms

Synonyms Comment Organism
FMNH2-dependent alkanesulfonate monooxygenase
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Escherichia coli
SsuD
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Escherichia coli
SsuE
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Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
FMNH2
-
Escherichia coli