Cloned (Comment) | Organism |
---|---|
expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
an alkanesulfonate + FMNH2 + O2 | Escherichia coli | the two-component alkanesulfonate monooxygenase system, with the flavin mononucleotide reductase, SsuE, being a part of it besides SsuD, utilizes reduced flavin as a substrate to catalyze a unique desulfonation reaction during times of sulfur starvation, protein-protein interactions are important in the mechanism of flavin transfer | an aldehyde + FMN + sulfite + H2O | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
an alkanesulfonate + FMNH2 + O2 | - |
Escherichia coli | an aldehyde + FMN + sulfite + H2O | - |
ir | |
an alkanesulfonate + FMNH2 + O2 | the two-component alkanesulfonate monooxygenase system, with the flavin mononucleotide reductase, SsuE, being a part of it besides SsuD, utilizes reduced flavin as a substrate to catalyze a unique desulfonation reaction during times of sulfur starvation, protein-protein interactions are important in the mechanism of flavin transfer | Escherichia coli | an aldehyde + FMN + sulfite + H2O | - |
ir | |
additional information | the enzyme interacts with the flavin mononucleotide reductase, SsuE, in a 1:1 monomeric association, mechanism of protein-protein interaction not leading to overall conformational changes in protein structure, overview | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SsuD | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMNH2 | - |
Escherichia coli |