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Literature summary for 1.14.14.5 extracted from

  • Abdurachim, K.; Ellis, H.R.
    Detection of protein-protein interactions in the alkanesulfonate monooxygenase system from Escherichia coli (2006), J. Bacteriol., 188, 8153-8159.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information steady-state kinetics Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
an alkanesulfonate + FMNH2 + O2 Escherichia coli the two-component alkanesulfonate monooxygenase system, with the flavin mononucleotide reductase, SsuE, being a part of it besides SsuD, utilizes reduced flavin as a substrate to catalyze a unique desulfonation reaction during times of sulfur starvation, protein-protein interactions are important in the mechanism of flavin transfer an aldehyde + FMN + sulfite + H2O
-
ir

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
an alkanesulfonate + FMNH2 + O2
-
Escherichia coli an aldehyde + FMN + sulfite + H2O
-
ir
an alkanesulfonate + FMNH2 + O2 the two-component alkanesulfonate monooxygenase system, with the flavin mononucleotide reductase, SsuE, being a part of it besides SsuD, utilizes reduced flavin as a substrate to catalyze a unique desulfonation reaction during times of sulfur starvation, protein-protein interactions are important in the mechanism of flavin transfer Escherichia coli an aldehyde + FMN + sulfite + H2O
-
ir
additional information the enzyme interacts with the flavin mononucleotide reductase, SsuE, in a 1:1 monomeric association, mechanism of protein-protein interaction not leading to overall conformational changes in protein structure, overview Escherichia coli ?
-
?

Synonyms

Synonyms Comment Organism
SsuD
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
FMNH2
-
Escherichia coli