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Literature summary for 1.14.14.5 extracted from

  • Gao, B.; Ellis, H.R.
    Mechanism of flavin reduction in the alkanesulfonate monooxygenase system (2007), Biochim. Biophys. Acta, 1774, 359-367.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene ssuD, the alkanesulfonate monooxygenase system, expressed from the ssuEADCB operon, is comprised of a flavin reductase encoded by ssuE and monooxygenase encoded by ssuD, ssuD expressionin strain BL21(DE3) Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information stopped-flow kinetics Escherichia coli
additional information
-
additional information rapid reaction kinetic analysis of SsuE-catalyzed flavin reduction using NADPH Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
an alkanesulfonate + FMNH2 + O2 Escherichia coli the enzyme is involved in scavenging sulfur from alkanesulfonates under sulfur starvation an aldehyde + FMN + sulfite + H2O
-
?
an alkanesulfonate + FMNH2 + O2 Escherichia coli the enzyme system is involved in scavenging sulfur from alkanesulfonates under sulfur starvation, overview an aldehyde + FMN + sulfite + H2O
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Reaction

Reaction Comment Organism Reaction ID
an alkanesulfonate + FMNH2 + O2 = an aldehyde + FMN + sulfite + H2O kinetic reaction mechanism, charge transfer complex formation with flavin mononucleotide reductase in the alkanesulfonate monooxygenase system Escherichia coli
an alkanesulfonate + FMNH2 + O2 = an aldehyde + FMN + sulfite + H2O mechanism of flavin reduction in the alkanesulfonate monooxygenase system, overview Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
an alkanesulfonate + FMNH2 + O2
-
Escherichia coli an aldehyde + FMN + sulfite + H2O
-
?
an alkanesulfonate + FMNH2 + O2 the enzyme is involved in scavenging sulfur from alkanesulfonates under sulfur starvation Escherichia coli an aldehyde + FMN + sulfite + H2O
-
?
an alkanesulfonate + FMNH2 + O2 the enzyme system is involved in scavenging sulfur from alkanesulfonates under sulfur starvation, overview Escherichia coli an aldehyde + FMN + sulfite + H2O
-
?
an alkanesulfonate + FMNH2 + O2 mechanism of flavin reduction in the alkanesulfonate monooxygenase system, the FMN reductase, SsuE, catalyzes the reduction of FMN by NADPH, and the reduced flavin is transferred to the monooxygenase, SsuD, overview Escherichia coli an aldehyde + FMN + sulfite + H2O
-
?
additional information mechanism of flavin reduction in the alkanesulfonate monooxygenase system, consisting of the alkanesulfonate monooxygenase and the flavin mononucleotide reductase, which catalyzes the reduction of FMN by NADPH, overview Escherichia coli ?
-
?

Synonyms

Synonyms Comment Organism
SsuD
-
Escherichia coli
SsuE
-
Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
FMNH2
-
Escherichia coli