Cloned (Comment) | Organism |
---|---|
gene ssuD, the alkanesulfonate monooxygenase system, expressed from the ssuEADCB operon, is comprised of a flavin reductase encoded by ssuE and monooxygenase encoded by ssuD, ssuD expressionin strain BL21(DE3) | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | stopped-flow kinetics | Escherichia coli | |
additional information | - |
additional information | rapid reaction kinetic analysis of SsuE-catalyzed flavin reduction using NADPH | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
an alkanesulfonate + FMNH2 + O2 | Escherichia coli | the enzyme is involved in scavenging sulfur from alkanesulfonates under sulfur starvation | an aldehyde + FMN + sulfite + H2O | - |
? | |
an alkanesulfonate + FMNH2 + O2 | Escherichia coli | the enzyme system is involved in scavenging sulfur from alkanesulfonates under sulfur starvation, overview | an aldehyde + FMN + sulfite + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
an alkanesulfonate + FMNH2 + O2 = an aldehyde + FMN + sulfite + H2O | kinetic reaction mechanism, charge transfer complex formation with flavin mononucleotide reductase in the alkanesulfonate monooxygenase system | Escherichia coli | |
an alkanesulfonate + FMNH2 + O2 = an aldehyde + FMN + sulfite + H2O | mechanism of flavin reduction in the alkanesulfonate monooxygenase system, overview | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
an alkanesulfonate + FMNH2 + O2 | - |
Escherichia coli | an aldehyde + FMN + sulfite + H2O | - |
? | |
an alkanesulfonate + FMNH2 + O2 | the enzyme is involved in scavenging sulfur from alkanesulfonates under sulfur starvation | Escherichia coli | an aldehyde + FMN + sulfite + H2O | - |
? | |
an alkanesulfonate + FMNH2 + O2 | the enzyme system is involved in scavenging sulfur from alkanesulfonates under sulfur starvation, overview | Escherichia coli | an aldehyde + FMN + sulfite + H2O | - |
? | |
an alkanesulfonate + FMNH2 + O2 | mechanism of flavin reduction in the alkanesulfonate monooxygenase system, the FMN reductase, SsuE, catalyzes the reduction of FMN by NADPH, and the reduced flavin is transferred to the monooxygenase, SsuD, overview | Escherichia coli | an aldehyde + FMN + sulfite + H2O | - |
? | |
additional information | mechanism of flavin reduction in the alkanesulfonate monooxygenase system, consisting of the alkanesulfonate monooxygenase and the flavin mononucleotide reductase, which catalyzes the reduction of FMN by NADPH, overview | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SsuD | - |
Escherichia coli |
SsuE | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMNH2 | - |
Escherichia coli |