Protein Variants | Comment | Organism |
---|---|---|
W66F | mutation changes midpoint potential from -361 mV for wild-type to -427 mV. Mutant displays 2.5fold lower activity when reaction is supported by flavoproteins or NADPH instead of tetrahydrofolate | Bacillus subtilis |
W66H | mutation changes midpoint potential from -361 mV for wild-type to -302 mV. Activity is similar to wild-type | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | in the presence of L-arginine and tetrahydrofolate, wild-type and mutants W66F and W66H exist in the typical Fe(III) high-spin configuration | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 reduced flavodoxin + 4 O2 | - |
Bacillus subtilis | 2 L-citrulline + 2 nitric oxide + 3 oxidized flavodoxin + 4 H2O | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
(6R)-L-erythro-5,6,7,8-tetrahydrobiopterin | - |
Bacillus subtilis | |
additional information | enzyme is able to use several different redox partners | Bacillus subtilis | |
NADPH | - |
Bacillus subtilis | |
tetrahydrofolate | - |
Bacillus subtilis |