Cloned (Comment) | Organism |
---|---|
expression in Saccharomyces cerevisiae | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
indole-3-acetaldoxime | pH 8.1, 29°C | Arabidopsis thaliana | |
0.156 | - |
(E)-4-hydroxyphenylacetaldoxime | pH 8.1, 29°C | Arabidopsis thaliana | |
0.556 | - |
(E)-phenylacetaldoxime | pH 8.1, 29°C | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | P48421 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E)-4-hydroxyphenylacetaldoxime + O2 + [reduced NADPH-hemoprotein reductase] | - |
Arabidopsis thaliana | 1-aci-nitro-2-(4-hydroxyphenyl)-ethane + H2O + [oxidized NADPH-hemoprotein reductase] | - |
? | |
(E)-phenylacetaldoxime + O2 + [reduced NADPH-hemoprotein reductase] | - |
Arabidopsis thaliana | 1-aci-nitro-2-phenylethane + H2O + [oxidized NADPH-hemoprotein reductase] | - |
? | |
indole-3-acetaldoxime + O2 + glutathione + [reduced NADPH-hemoprotein reductase] | - |
Arabidopsis thaliana | 1-aci-nitro-2-indolyl-ethane + H2O + [oxidized NADPH-hemoprotein reductase] | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP83A1 | - |
Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.42 | - |
(E)-phenylacetaldoxime | pH 8.1, 29°C | Arabidopsis thaliana | |
0.43 | - |
(E)-4-hydroxyphenylacetaldoxime | pH 8.1, 29°C | Arabidopsis thaliana | |
2.33 | - |
indole-3-acetaldoxime | pH 8.1, 29°C | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
physiological function | aliphatic oximes derived from chain-elongated homologs of methionine are efficiently metabolized by isoform CYP83A1, whereas CYP83B1 metabolizes these substrates with very low efficiency. Aromatic oximes derived from phenylalanine, tryptophan, and tyrosine are metabolized by both enzymes, although CYP83B1 has higher affinity for these substrates than CYP83A1 | Arabidopsis thaliana |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.75 | - |
(E)-phenylacetaldoxime | pH 8.1, 29°C | Arabidopsis thaliana | |
2.76 | - |
(E)-4-hydroxyphenylacetaldoxime | pH 8.1, 29°C | Arabidopsis thaliana | |
15 | - |
indole-3-acetaldoxime | pH 8.1, 29°C | Arabidopsis thaliana |