Any feedback?
Literature summary for 1.14.14.40 extracted from
- Cytochrome P450 CYP79A2 from Arabidopsis thaliana L. catalyzes the conversion of L-phenylalanine to phenylacetaldoxime in the biosynthesis of benzylglucosinolate (2000), J. Biol. Chem., 275, 14659-14666.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| glutathione | reduced glutathione, final concentration of 3 mM, slight stimulation | Arabidopsis thaliana |  |
| reduced glutathione | addition of reduced glutathione to a final concentration of 3 mM stimulates aldoxime production | Arabidopsis thaliana | |
| Tween 80 | addition of Tween 80 to a final concentration of 0.083% (v/v) results in a 1.5fold increase in aldoxime production | Arabidopsis thaliana | |
| Tween 80 | final concentration of 0.083% (v/v), 1.5fold increase in aldoxime production | Arabidopsis thaliana | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli JM109 cells | Arabidopsis thaliana |
| expression in Escherichia coli | Arabidopsis thaliana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0067 | - |
L-phenylalanine | pH 7.5, 26°C | Arabidopsis thaliana | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
x * 60000, SDS-PAGE | Arabidopsis thaliana |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q9FLC8 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-phenylalanine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase] | - |
Arabidopsis thaliana | (E)-phenylacetaldoxime + 2 [oxidized NADPH-hemoprotein reductase] + CO2 + 3 H2O | overall reaction | ? | |
| L-phenylalanine + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 | - |
Arabidopsis thaliana | (E)-phenylacetaldoxime + 2 [oxidized NADPH-hemoprotein reductase] + CO2 + 3 H2O | - |
? | |
| additional information | neither L-tyrosine, L-tryptophan, L-methionine, nor DL-homophenylalanine are metabolized by CYP79A2 | Arabidopsis thaliana | ? | - |
? | |
| additional information | no substrates: L-tyrosine, L-tryptophan, L-methionine, or DL-homophenylalanine | Arabidopsis thaliana | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 60000, SDS-PAGE | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CYP79A2 | - |
Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Arabidopsis thaliana |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
25% of maximum activity | Arabidopsis thaliana |
| 6 | 7.9 | compared with the activity at pH 7.5, the activity of chimeric CYP79A2 is 25% at pH 6.0, 50% at pH 6.5, 80% at pH 7.0, and 70% at pH 7.9 | Arabidopsis thaliana |
| 7.9 | - |
70% of maximum activity | Arabidopsis thaliana |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome P450 | - |
Arabidopsis thaliana | |
| NADPH | - |
Arabidopsis thaliana | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | CYP79A2 is involved in the biosynthesis of benzylglucosinolate | Arabidopsis thaliana |
| physiological function | enzyme is an N-hydroxylase converting L-phenylalanine into phenylacetaldoxime, the precursor of benzylglucosinolate. Transgenic Arabidopsis thaliana constitutively expressing CYP79A2 accumulate high levels of benzylglucosinolate | Arabidopsis thaliana |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
