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Literature summary for 1.14.14.37 extracted from

  • Kahn, R.A.; Bak, S.; Svendsen, I.; Halkier, B.A.; Moller, B.L.
    Isolation and reconstitution of cytochrome P450ox and in vitro reconstitution of the entire biosynthetic pathway of the cyanogenic glucoside dhurrin from sorghum (1997), Plant Physiol., 115, 1661-1670.
    View publication on PubMedView publication on EuropePMC
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Application

Application Comment Organism
biotechnology in vitro reconstitution of the entire dhurrin biosynthetic pathway from tyrosine is accomplished by the insertion of CYP79 (tyrosine N-hydroxylase), P450ox, and NADPH-P450 oxidoreductase in lipid micelles in the presence of uridine diphosphate glucose glucosyltransferase Sorghum bicolor

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 x * 55000, SDS-PAGE Sorghum bicolor

Organism

Organism UniProt Comment Textmining
Sorghum bicolor O48958
-
-

Renatured (Commentary)

Renatured (Comment) Organism
reconstitution with NADPH-P450 oxidoreductase in micelles of L-alpha-dilauroyl phosphatidylcholine Sorghum bicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E)-4-Hydroxyphenylacetaldehyde oxime
-
 Sorghum bicolor (Z)-4-Hydroxyphenylacetaldehyde oxime
-
 ?
(E)-4-hydroxyphenylacetaldehyde oxime + [reduced NADPH-hemoprotein reductase] + O2
-
 Sorghum bicolor (S)-4-hydroxymandelonitrile + [oxidized NADPH-hemoprotein reductase] + 2 H2O overall reaction ?
(Z)-4-hydroxyphenylacetaldehyde oxime
-
 Sorghum bicolor 4-hydroxyphenylacetonitrile + H2O
-
 ?
4-hydroxyphenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2
-
 Sorghum bicolor (S)-4-hydroxymandelonitrile + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 55000, SDS-PAGE Sorghum bicolor

General Information

General Information Comment Organism
physiological function the enzyme is a multifunctional P450 catalyzing dehydration of (Z)-4-hydroxyphenylacetaldoxime to 4-hydroxyphenylacetonitrile and C-hydroxylation of 4-hydroxyphenylacetonitrile during biosynthesis of the cyanogenic glucoside beta-D-glucopyranosyloxy-(S)-4-hydroxymandelonitrile (dhurrin) Sorghum bicolor