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Literature summary for 1.14.14.33 extracted from
- System of oligonucleotide primers for detection and amplification of the emoA gene encoding bacterial ethylenediaminetetraacetate monooxygenase (2008), Appl. Biochem. Microbiol., 44, 361-365.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| FAD | stimulation | Stenotrophomonas chelatiphaga |  |
| FMN | stimulation | Chelativorans multitrophicus | |
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | design of a system of primers to obtain emoA gene fragments approximately 750 bp long for bacterial destructors of EDTA. The system can be effectively used for detecting the emoA gene in representatives of Alpha- and Gammaproteobacteria | Chelativorans oligotrophicus |
| analysis | design of a system of primers to obtain emoA gene fragments approximately 750 bp long for bacterial destructors of EDTA. The system can be effectively used for detecting the emoA gene in representatives of Alpha- and Gammaproteobacteria | Chelativorans multitrophicus |
| analysis | design of a system of primers to obtain emoA gene fragments approximately 750 bp long for bacterial destructors of EDTA. The system can be effectively used for detecting the emoA gene in representatives of Alpha- and Gammaproteobacteria | Stenotrophomonas chelatiphaga |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ethylenediaminetetraacetate + 2 FMNH2 + 2 O2 | Chelativorans oligotrophicus | - |
ethylenediamine-N,N'-diacetate + 2 glyoxylate + 2 FMN + 2 H2O | overall reaction | ? | |
| ethylenediaminetetraacetate + 2 FMNH2 + 2 O2 | Chelativorans multitrophicus | - |
ethylenediamine-N,N'-diacetate + 2 glyoxylate + 2 FMN + 2 H2O | overall reaction | ? | |
| ethylenediaminetetraacetate + 2 FMNH2 + 2 O2 | Stenotrophomonas chelatiphaga | - |
ethylenediamine-N,N'-diacetate + 2 glyoxylate + 2 FMN + 2 H2O | overall reaction | ? | |
| ethylenediaminetetraacetate + 2 FMNH2 + 2 O2 | Chelativorans oligotrophicus LPM-4 | - |
ethylenediamine-N,N'-diacetate + 2 glyoxylate + 2 FMN + 2 H2O | overall reaction | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chelativorans multitrophicus | A7LKG7 | - |
- |
| Chelativorans oligotrophicus | A7LIY3 | - |
- |
| Chelativorans oligotrophicus LPM-4 | A7LIY3 | - |
- |
| Stenotrophomonas chelatiphaga | A7LKG8 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 16 | - |
presence of NADH2, pH 8.0, 30°C | Stenotrophomonas chelatiphaga |
| 17 | - |
presence of NADH2 and FMNH2, pH 8.0, 30°C | Stenotrophomonas chelatiphaga |
| 29 | - |
presence of NADH2 and FADH2, pH 8.0, 30°C | Stenotrophomonas chelatiphaga |
| 30 | - |
presence of NADH, pH 8.0, 30°C | Chelativorans multitrophicus |
| 70 | - |
presence of NADH and FADH2, pH 8.0, 30°C | Chelativorans multitrophicus |
| 80 | - |
presence of NADH, pH 8.0, 30°C | Chelativorans oligotrophicus |
| 81 | - |
presence of NADH and FADH2, pH 8.0, 30°C | Chelativorans oligotrophicus |
| 107 | - |
presence of NADH and FMNH2, pH 8.0, 30°C | Chelativorans multitrophicus |
| 156 | - |
presence of NADH and FMNH2, pH 8.0, 30°C | Chelativorans oligotrophicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ethylenediaminetetraacetate + 2 FADH2 + 2 O2 | - |
Chelativorans oligotrophicus | ethylenediamine-N,N'-diacetate + 2 glyoxylate + 2 FAD + 2 H2O | overall reaction | ? | |
| ethylenediaminetetraacetate + 2 FADH2 + 2 O2 | - |
Chelativorans multitrophicus | ethylenediamine-N,N'-diacetate + 2 glyoxylate + 2 FAD + 2 H2O | overall reaction | ? | |
| ethylenediaminetetraacetate + 2 FADH2 + 2 O2 | - |
Stenotrophomonas chelatiphaga | ethylenediamine-N,N'-diacetate + 2 glyoxylate + 2 FAD + 2 H2O | overall reaction | ? | |
| ethylenediaminetetraacetate + 2 FADH2 + 2 O2 | - |
Chelativorans oligotrophicus LPM-4 | ethylenediamine-N,N'-diacetate + 2 glyoxylate + 2 FAD + 2 H2O | overall reaction | ? | |
| ethylenediaminetetraacetate + 2 FMNH2 + 2 O2 | - |
Chelativorans oligotrophicus | ethylenediamine-N,N'-diacetate + 2 glyoxylate + 2 FMN + 2 H2O | overall reaction | ? | |
| ethylenediaminetetraacetate + 2 FMNH2 + 2 O2 | - |
Chelativorans multitrophicus | ethylenediamine-N,N'-diacetate + 2 glyoxylate + 2 FMN + 2 H2O | overall reaction | ? | |
| ethylenediaminetetraacetate + 2 FMNH2 + 2 O2 | - |
Stenotrophomonas chelatiphaga | ethylenediamine-N,N'-diacetate + 2 glyoxylate + 2 FMN + 2 H2O | overall reaction | ? | |
| ethylenediaminetetraacetate + 2 FMNH2 + 2 O2 | - |
Chelativorans oligotrophicus LPM-4 | ethylenediamine-N,N'-diacetate + 2 glyoxylate + 2 FMN + 2 H2O | overall reaction | ? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EmoA | - |
Chelativorans oligotrophicus |
| EmoA | - |
Chelativorans multitrophicus |
| EmoA | - |
Stenotrophomonas chelatiphaga |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMNH2 | - |
Chelativorans oligotrophicus | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | enzyme catalyzes the first reaction of EDTA decomposition by cell extracts, an NADH-dependent process that is accompanied by O2 uptake | Chelativorans oligotrophicus |
| physiological function | enzyme catalyzes the first reaction of EDTA decomposition by cell extracts, an NADH-dependent process that is accompanied by O2 uptake | Chelativorans multitrophicus |
| physiological function | enzyme catalyzes the first reaction of EDTA decomposition by cell extracts, an NADH2-dependent process that is accompanied by O2 uptake | Stenotrophomonas chelatiphaga |
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