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Literature summary for 1.14.14.3 extracted from
- Complex formation between Vibrio harveyi luciferase and monomeric NADPH:FMN oxidoreductase (2003), Biochemistry, 42, 529-534.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | enzyme activities in complex formation, kinetics, dissociation constants | Vibrio harveyi |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Vibrio harveyi | complex formation in a 1:1 molar ratio between monomeric, but not dimeric, NADPH:FMN oxidoreductase FRP and luciferase for direct transfer of cofactor FMNH2 | ? | - |
? |  |
| tetradecanal + FMNH2 + O2 | Vibrio harveyi | - |
tetradecanoate + FMN + H2O + hn | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vibrio harveyi | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.105 | - |
substrate tetradecanal, uncoupled enzyme | Vibrio harveyi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | complex formation in a 1:1 molar ratio between monomeric, but not dimeric, NADPH:FMN oxidoreductase FRP and luciferase for direct transfer of cofactor FMNH2 | Vibrio harveyi | ? | - |
? | |
| RCHO + FMNH2 + O2 | - |
Vibrio harveyi | RCOOH + FMN + H2O + hn | - |
ir | |
| tetradecanal + FMNH2 + O2 | - |
Vibrio harveyi | tetradecanoate + FMN + H2O + hn | - |
ir | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| luciferase | - |
Vibrio harveyi |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 23 | - |
assay at | Vibrio harveyi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Vibrio harveyi |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMNH2 | - |
Vibrio harveyi | |
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Release 2023.1 (January 2023)
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