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Literature summary for 1.14.14.3 extracted from
- Mutational analysis of the subunit interface of Vibrio harveyi bacterial luciferase (2002), Biochemistry, 41, 3906-3915.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of wild-type and mutant enzymes in Escherichia coli | Vibrio harveyi |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A81H | site-directed mutagenesis, residue of the alpha-subunit, mutant shows 13% of wild-type activity | Vibrio harveyi |
| H81A | site-directed mutagenesis, residue of the beta-subunit, mutant shows 59% of wild-type activity | Vibrio harveyi |
| H81A/E89D | site-directed mutagenesis, residues of the beta-subunit, mutant shows 13% of wild-type activity | Vibrio harveyi |
| H82A | site-directed mutagenesis, residue of the beta-subunit, mutant shows 22% of wild-type activity | Vibrio harveyi |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Urea | denaturation curve, thermodynamics, wild-type and mutants, overview | Vibrio harveyi |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | detailed reaction and folding kinetics, thermodynamics | Vibrio harveyi |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 75430 | - |
wild-type enzyme, analytical ultracentrifugation | Vibrio harveyi |
| 76360 | - |
mutant beta-H82A, analytical ultracentrifugation | Vibrio harveyi |
| 76740 | - |
mutant beta-H81A, analytical ultracentrifugation | Vibrio harveyi |
| 76750 | - |
mutant beta-H81A/E89D, analytical ultracentrifugation | Vibrio harveyi |
| 76860 | - |
mutant alpha-A81H, analytical ultracentrifugation | Vibrio harveyi |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vibrio harveyi | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| RCHO + FMNH2 + O2 | - |
Vibrio harveyi | RCOOH + FMN + H2O + hn | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | analysis of subunit interface structure and role in the conformational stability of the heterodimeric enzyme, the beta-sunbunit can self-associate to form a stable but inactive homodimer, unfolding in a four-state mechanism | Vibrio harveyi |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| bacterial luciferase | - |
Vibrio harveyi |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Vibrio harveyi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Vibrio harveyi |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMNH2 | - |
Vibrio harveyi | |
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Release 2023.1 (January 2023)
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