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Literature summary for 1.14.14.28 extracted from

  • Li, L.; Liu, X.; Yang, W.; Xu, F.; Wang, W.; Feng, L.; Bartlam, M.; Wang, L.; Rao, Z.
    Crystal structure of long-chain alkane monooxygenase (LadA) in complex with coenzyme FMN: unveiling the long-chain alkane hydroxylase (2008), J. Mol. Biol., 376, 453-465.
    View publication on PubMed
Search for more literature for 1.14.14.28

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Geobacillus thermodenitrificans

Crystallization (Commentary)

Crystallization (Comment) Organism
crystals of LadA and the LadA:FMN complex are grown using the hanging-drop vapor-diffusion method at 18°C, crystal structure of the enzyme in the apoenzyme form and its complex with FMNH2 Geobacillus thermodenitrificans

Protein Variants

Protein Variants Comment Organism
C14A point mutation completely abolishes the catalytic activity Geobacillus thermodenitrificans
H17F the mutant maintains the same dimeric form as the wild type, point mutation completely abolishes the catalytic activity Geobacillus thermodenitrificans
H311F the mutant maintains the same dimeric form as the wild type, point mutation completely abolishes the catalytic activity Geobacillus thermodenitrificans
Q79L the mutant maintains the same dimeric form as the wild type, point mutation completely abolishes the catalytic activity Geobacillus thermodenitrificans
T63F the mutant maintains the same dimeric form as the wild type, point mutation completely abolishes the catalytic activity Geobacillus thermodenitrificans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50466
-
 2 * 50466, calculated from sequence Geobacillus thermodenitrificans
105000
-
 gel filtration Geobacillus thermodenitrificans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a long-chain alkane + FMNH2 + O2 Geobacillus thermodenitrificans
-
 a long-chain primary alcohol + FMN + H2O
-
 ?
a long-chain alkane + FMNH2 + O2 Geobacillus thermodenitrificans NG80-2
-
 a long-chain primary alcohol + FMN + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Geobacillus thermodenitrificans A4IU28
-
-
Geobacillus thermodenitrificans NG80-2 A4IU28
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Geobacillus thermodenitrificans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a long-chain alkane + FMNH2 + O2
-
 Geobacillus thermodenitrificans a long-chain primary alcohol + FMN + H2O
-
 ?
a long-chain alkane + FMNH2 + O2 the enzyme is capable of converting alkanes ranging from C15 to C36 into their corresponding primary alcohols Geobacillus thermodenitrificans a long-chain primary alcohol + FMN + H2O
-
 ?
a long-chain alkane + FMNH2 + O2
-
 Geobacillus thermodenitrificans NG80-2 a long-chain primary alcohol + FMN + H2O
-
 ?
a long-chain alkane + FMNH2 + O2 the enzyme is capable of converting alkanes ranging from C15 to C36 into their corresponding primary alcohols Geobacillus thermodenitrificans NG80-2 a long-chain primary alcohol + FMN + H2O
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 50466, calculated from sequence Geobacillus thermodenitrificans

Synonyms

Synonyms Comment Organism
LADA
-
 Geobacillus thermodenitrificans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Geobacillus thermodenitrificans

Cofactor

Cofactor Comment Organism Structure
FMNH2
-
 Geobacillus thermodenitrificans