Literature summary for 1.14.14.25 extracted from

Mast, N.; Andersson, U.; Nakayama, K.; Bjorkhem, I.; Pikuleva, I.A.
Expression of human cytochrome P450 46A1 in Escherichia coli: effects of N- and C-terminal modifications (2004), Arch. Biochem. Biophys., 428, 99-108.

Search for more literature for 1.14.14.25

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli, wild-type and trucation mutatants. All four mutants lack the N-terminal transmembrane region (residues 327), and, in addition, DELTA46A1 has a 4 His-tag fused to the C-terminus, HDELTA46A1 has the N-terminal 4 His-tag, HDELTA46A1DELTA has a 4 His-tag at the N-terminus and does not contain a proline-rich region at the C-terminus (residues 494499), and DELTA46A1DELTA lacks the C-terminal proline-rich region	Homo sapiens

Cloned (Comment)

Organism

expression in Escherichia coli, wild-type and trucation mutatants. All four mutants lack the N-terminal transmembrane region (residues 327), and, in addition, DELTA46A1 has a 4 His-tag fused to the C-terminus, HDELTA46A1 has the N-terminal 4 His-tag, HDELTA46A1DELTA has a 4 His-tag at the N-terminus and does not contain a proline-rich region at the C-terminus (residues 494499), and DELTA46A1DELTA lacks the C-terminal proline-rich region

Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	four truncation mutants: all lack the N-terminal transmembrane region (residues 3 27), and, in addition, DELTA46A1 has a 4 His-tag fused to the C-terminus, HDELTA46A1 has the N-terminal 4 His-tag, HDELTA46A1DELTA has a 4 His-tag at the N-terminus and does not contain a proline-rich region at the C-terminus (residues 494 499), and DELTA46A1DELTA lacks the C-terminal proline-rich region. Truncated enzymes have moderately decreased catalytic efficiencies for either cholesterol or 24S-hydroxycholesterol or both, whereas their substrate-binding constants are either unchanged or decreased 2fold.The two forms, DELTA64A1DELTA and HDELTA46A1DELTA both lacking the C-terminal proline-rich region are good candidates for future crystallographic studies because they contain only 0.3 0.8% of high molecular weight aggregates and their catalytic efficiencies are decreased no more than 2.3fold	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	Km-value of truncated enzyme forms	Homo sapiens
0.0022	-	(24S)-24-hydroxycholesterol	full length enzyme	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	truncated enzymes are distributed at about a 1:1 ratio between the membrane fraction and the cytosol in low ionic strength buffer, when expressed in Escherichia coli	Homo sapiens	5829	-
membrane	truncated enzymes are distributed at about a 1:1 ratio between the membrane fraction and the cytosol in low ionic strength buffer, when expressed in Escherichia coli	Homo sapiens	16020	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
49000	-	1 * 49000, truncated enzyme form, SDS-PAGE	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
truncated enzyme forms	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
(24S)-24-hydroxycholesterol + [reduced NADPH-hemoprotein reductase] + O2	-	Homo sapiens	24,25-dihydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O	-	?
(24S)-24-hydroxycholesterol + [reduced NADPH-hemoprotein reductase] + O2	-	Homo sapiens	24,27-dihydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O	-	?
cholesterol + [reduced NADPH-hemoprotein reductase] + H+ + O2	-	Homo sapiens	(24S)-24-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 49000, truncated enzyme form, SDS-PAGE	Homo sapiens

Synonyms

Synonyms	Comment	Organism
cholesterol 24-monooxygenase	-	Homo sapiens
cholesterol 24S-hydroxylase	-	Homo sapiens
CYP46	-	Homo sapiens
CYP46A1	-	Homo sapiens
cytochrome P-450 46A1	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
additional information	-	additional information	turnover-numbers of truncated enzyme forms	Homo sapiens
0.00117	-	(24S)-24-hydroxycholesterol	full length enzyme	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
cytochrome P-450	-	Homo sapiens
NADPH	-	Homo sapiens