Activating Compound | Comment | Organism | Structure |
---|---|---|---|
KCl | or NaCl, required. Optimum concentration 100 mM | Pseudonocardia autotrophica | |
NaCl | or KCl, required. Optimum concentration 100 mM | Pseudonocardia autotrophica | |
partially methylated beta-cyclodextrin | activity increases approximately 10fold when partially methylated beta-cyclodextrin is present in the reaction at a final concentration of 0.05% | Pseudonocardia autotrophica |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudonocardia autotrophica |
Protein Variants | Comment | Organism |
---|---|---|
E216A | 2- to 3fold increase in hydroxylase activity | Pseudonocardia autotrophica |
E384R | 2- to 3fold increase in hydroxylase activity | Pseudonocardia autotrophica |
T70R | 2- to 3fold increase in hydroxylase activity | Pseudonocardia autotrophica |
T70R/V156L/E216M/E384R | 21fold increase in hydroxylase activity | Pseudonocardia autotrophica |
T70R/V156S/E216A/E384R | 7.9fold increase in hydroxylase activity | Pseudonocardia autotrophica |
V156S | 2- to 3fold increase in hydroxylase activity | Pseudonocardia autotrophica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0071 | - |
25-hydroxy-vitamin D3 | 30°C, pH not specified in the publication | Pseudonocardia autotrophica | |
0.0135 | - |
vitamin D3 | 30°C, pH not specified in the publication | Pseudonocardia autotrophica | |
0.032 | - |
vitamin D3 | mutant T70R/V156L/E216M/E384R, 30°C, pH not specified in the publication | Pseudonocardia autotrophica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44368 | - |
x * 44368, calculated | Pseudonocardia autotrophica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudonocardia autotrophica | C4B644 | - |
- |
Pseudonocardia autotrophica NBRC 12743 | C4B644 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
vitamin D3 + O2 + NADPH + H+ | - |
Pseudonocardia autotrophica | 1alpha, 25-dihydroxy-vitamin D3 + NADP+ + H2O | major products are 25-hydroxy-vitamin D3 and 1alpha, 25-dihydroxy-vitamin D3, plus minor amounts of 26-hydroxy-vitamin D3 | ? | |
vitamin D3 + O2 + NADPH + H+ | - |
Pseudonocardia autotrophica NBRC 12743 | 1alpha, 25-dihydroxy-vitamin D3 + NADP+ + H2O | major products are 25-hydroxy-vitamin D3 and 1alpha, 25-dihydroxy-vitamin D3, plus minor amounts of 26-hydroxy-vitamin D3 | ? | |
vitamin D3 + O2 + NADPH + H+ | - |
Pseudonocardia autotrophica | 25-hydroxy-vitamin D3 + NADP+ + H2O | major products are 25-hydroxy-vitamin D3 and 1alpha, 25-dihydroxy-vitamin D3, plus minor amounts of 26-hydroxy-vitamin D3. Data indicate that Vdh first hydroxylates the C-25 position of vitamion D3 and subsequently hydroxylates the C1alpha position of 25-hydroxy-vitamin D3 | ? | |
vitamin D3 + O2 + NADPH + H+ | - |
Pseudonocardia autotrophica NBRC 12743 | 25-hydroxy-vitamin D3 + NADP+ + H2O | major products are 25-hydroxy-vitamin D3 and 1alpha, 25-dihydroxy-vitamin D3, plus minor amounts of 26-hydroxy-vitamin D3. Data indicate that Vdh first hydroxylates the C-25 position of vitamion D3 and subsequently hydroxylates the C1alpha position of 25-hydroxy-vitamin D3 | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 44368, calculated | Pseudonocardia autotrophica |
Synonyms | Comment | Organism |
---|---|---|
VDH | - |
Pseudonocardia autotrophica |
vitamin D3 hydroxylase | - |
Pseudonocardia autotrophica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Pseudonocardia autotrophica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | - |
Pseudonocardia autotrophica |