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Literature summary for 1.14.14.23 extracted from
- Conversion of 7-dehydrocholesterol to 7-ketocholesterol is catalyzed by human cytochrome P450 7A1 and occurs by direct oxidation without an epoxide intermediate (2011), J. Biol. Chem., 286, 33021-33028.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | in inborn error of metabolism known as Smith-Lemli-Opitz syndrome, 7-oxocholesterol levels may also be elevated in tissue and fluids as a result of CYP7A1 oxidation of 7-dehydrocholesterol | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0011 | - |
7-dehydrocholesterol | pH 7.4, 30°C | Homo sapiens |  |
| 0.0018 | - |
lathosterol | 7-oxocholestanol formation, pH 7.4, 30°C | Homo sapiens | |
| 0.0021 | - |
lathosterol | epoxide formation, pH 7.4, 30°C | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Homo sapiens | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7-dehydrocholesterol + NADPH + H+ | Homo sapiens | - |
7-oxocholesterol + NADP+ + H2O | minor product: 7alpha,8alpha-epoxide | ? | |
| lathosterol + NADPH + H+ | Homo sapiens | i.e. DELTA-dihydro-7-dehydrocholesterol | 7-oxocholestanol + cholestanol-7alpha,8alpha-epoxide + NADP+ + H2O | about 1:2 ratio of 7-oxo and epoxide products, the epoxide does not rearrange to the ketone | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7-dehydrocholesterol + NADPH + H+ | - |
Homo sapiens | 7-oxocholesterol + NADP+ + H2O | minor product: 7alpha,8alpha-epoxide | ? | |
| 7-dehydrocholesterol + NADPH + H+ | - |
Homo sapiens | 7-oxocholesterol + NADP+ + H2O | reaction with 7-[2H1]dehydrocholesterol yields complete migration of deuterium in the product 7-oxocholesterol. Minor product: 7alpha8alpha-epoxide | ? | |
| lathosterol + NADPH + H+ | i.e. DELTA-dihydro-7-dehydrocholesterol | Homo sapiens | 7-oxocholestanol + cholestanol-7alpha,8alpha-epoxide + NADP+ + H2O | about 1:2 ratio of 7-oxo and epoxide products, the epoxide does not rearrange to the ketone | ? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CYP7A1 | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
7-dehydrocholesterol | pH 7.4, 30°C | Homo sapiens | |
| 0.06 | - |
lathosterol | 7-oxocholestanol formation, pH 7.4, 30°C | Homo sapiens | |
| 0.12 | - |
lathosterol | epoxide formation, pH 7.4, 30°C | Homo sapiens | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 30 | - |
7-dehydrocholesterol | pH 7.4, 30°C | Homo sapiens | |
| 30 | - |
lathosterol | 7-oxocholestanol formation, pH 7.4, 30°C | Homo sapiens | |
| 50 | - |
lathosterol | epoxide formation, pH 7.4, 30°C | Homo sapiens | |
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Release 2023.1 (January 2023)
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