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Literature summary for 1.14.14.23 extracted from
- Regulation of cholesterol 7alpha-hydroxylase in the liver. Purification of cholesterol 7alpha-hydroxylase and the immunochemical evidence for the induction of cholesterol 7alpha-hydroxylase by cholestyramine and circadian rhythm (1990), J. Biol. Chem., 265, 3889-3897.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | stimulates | Rattus norvegicus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.031 | - |
cholesterol | isoenzyme II | Rattus norvegicus | |
| 0.052 | - |
cholesterol | isoenzyme I | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Rattus norvegicus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53000 | - |
x * 53000, SDS-PAGE | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 2 isoenzymes: I and II | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 36.7 | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cholesterol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Rattus norvegicus | 7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 53000, SDS-PAGE | Rattus norvegicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0417 | - |
cholesterol | isoenzyme I | Rattus norvegicus | |
| 0.0778 | - |
cholesterol | isoenzyme II | Rattus norvegicus | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | cytochrome P-450 dependent enzyme | Rattus norvegicus | |
| heme | 8 nmol of heme per mg of protein | Rattus norvegicus | |
| NADPH | - |
Rattus norvegicus | |
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Release 2023.1 (January 2023)
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