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Literature summary for 1.14.14.23 extracted from

  • Nguyen, L.B.; Shefer, S.; Salen, G.; Ness, G.; Tanaka, R.D.; Packin, V.; Thomas, P.; Shore, V.; Batta, A.
    Purification of cholesterol 7alpha-hydroxylase from human and rat liver and production of inhibiting polyclonal antibodies (1990), J. Biol. Chem., 265, 4541-4546.
    View publication on PubMed

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Homo sapiens
-
-
microsome
-
Rattus norvegicus
-
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens
-
Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Homo sapiens
-
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00015
-
-
Homo sapiens
0.01
-
-
Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
-
Homo sapiens 7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
-
Rattus norvegicus 7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Cofactor

Cofactor Comment Organism Structure
heme cytochrome P-450 dependent enzyme Homo sapiens
heme cytochrome P-450 dependent enzyme Rattus norvegicus
NADPH
-
Homo sapiens
NADPH
-
Rattus norvegicus