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Literature summary for 1.14.14.22 extracted from

  • Izumi, Y.; Ohshiro, T.
    Purification and characterization of enzymes involved in desulfurization of dibezothiophene in fossil fuels (2001), J. Mol. Catal. B, 11, 1061-1064.
No PubMed abstract available

Crystallization (Commentary)

Crystallization (Comment) Organism
purified enzyme DszA, hanging drop vapour diffusion method, using a reservoir suliton containing 12% polyethylene glycol 400 and 0.2 M CaCl2, 4┬░C, X-ray diffraction structure determination and analysis Rhodococcus erythropolis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
2 * 50000, SDS-PAGE Rhodococcus erythropolis
97000
-
gel filtration Rhodococcus erythropolis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 Rhodococcus erythropolis
-
2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O
-
?
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 Rhodococcus erythropolis D-1
-
2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O
-
?

Organism

Organism UniProt Comment Textmining
Rhodococcus erythropolis Q6WE15 gene dszA
-
Rhodococcus erythropolis D-1 Q6WE15 gene dszA
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme to homogeneity by anion exchange and hydrophobic interaction chromatography, and gel filtration Rhodococcus erythropolis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2
-
Rhodococcus erythropolis 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O
-
?
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 best substrate Rhodococcus erythropolis 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O
-
?
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2
-
Rhodococcus erythropolis D-1 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O
-
?
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 best substrate Rhodococcus erythropolis D-1 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O
-
?
dibenz[c,e][1,2]oxathiin 6,6-dioxide + 2 FMNH2 + O2 i.e. sultone Rhodococcus erythropolis 2,2'-dihydroxybiphenyl + 2 FMN + H2O
-
?
dibenz[c,e][1,2]oxathiin 6,6-dioxide + 2 FMNH2 + O2 i.e. sultone Rhodococcus erythropolis D-1 2,2'-dihydroxybiphenyl + 2 FMN + H2O
-
?
additional information no activity with dibenzothiophene and 2'-hydroxybiphenyl 2-sulfinic acid. DszA may recognize the sulfone moiety within the structure of DBT sulfone and sultone Rhodococcus erythropolis ?
-
?
additional information no activity with dibenzothiophene and 2'-hydroxybiphenyl 2-sulfinic acid. DszA may recognize the sulfone moiety within the structure of DBT sulfone and sultone Rhodococcus erythropolis D-1 ?
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 50000, SDS-PAGE Rhodococcus erythropolis

Synonyms

Synonyms Comment Organism
DBT sulfone monooxygenase
-
Rhodococcus erythropolis
dibenzothiophene sulfone monooxygenase
-
Rhodococcus erythropolis
dszA
-
Rhodococcus erythropolis

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
35
-
-
Rhodococcus erythropolis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Rhodococcus erythropolis

Cofactor

Cofactor Comment Organism Structure
FMNH2
-
Rhodococcus erythropolis

General Information

General Information Comment Organism
metabolism the enzyme is involved in the pathway of microbial dibenzothiophene desulfurization, overview Rhodococcus erythropolis