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Literature summary for 1.14.14.22 extracted from

  • Konishi, J.; Ishii, Y.; Onaka, T.; Ohta, Y.; Suzuki, M.; Maruhashi, K.
    Purification and characterization of dibenzothiophene sulfone monooxygenase and FMN-dependent NADH oxidoreductase from the thermophilic bacterium Paenibacillus sp. strain A11-2 (2000), J. Biosci. Bioeng., 90, 607-613.
    View publication on PubMed

Application

Application Comment Organism
additional information TdsA shows higher activity toward bulkier substrates than its mesophilic counterpart, DszA. These properties suggest the applicability of biodesulfmizatfon to the processing of actual petroleum fractions Paenibacillus sp.

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Paenibacillus sp.
4-chloromercuribenzoic acid
-
Paenibacillus sp.
5,5'-dithio-bis-2-nitrobenzoic acid
-
Paenibacillus sp.
Ca2+
-
Paenibacillus sp.
Co2+
-
Paenibacillus sp.
Cu2+ strong inhibition Paenibacillus sp.
EDTA
-
Paenibacillus sp.
iodoacetic acid
-
Paenibacillus sp.
Mn2+
-
Paenibacillus sp.
additional information no inhibition by 2-hydroxybiphenyl Paenibacillus sp.
N-ethylmaleimide
-
Paenibacillus sp.
Na2SO4
-
Paenibacillus sp.
sulfate slightly inhibiting Paenibacillus sp.
Zn2+
-
Paenibacillus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ slight activation Paenibacillus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
2 * 48000, SDS-PAGE Paenibacillus sp.
120000
-
gel filtration Paenibacillus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 Paenibacillus sp.
-
2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O
-
?
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 Paenibacillus sp. A11-2
-
2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O
-
?

Organism

Organism UniProt Comment Textmining
Paenibacillus sp. Q9LBX4 gene tdsA
-
Paenibacillus sp. A11-2 Q9LBX4 gene tdsA
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 47.6fold by anion exchange and hydrophobic interaction chromatography, followed by another different step of anion exchange chromatography and hydroxyapatite chromatography Paenibacillus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
6
-
purified enzyme, pH 7.0, 50┬░C, substrate 3,6-dimethyldibenzothiophene-5,5-dioxide Paenibacillus sp.
12.4
-
purified enzyme, pH 7.0, 50┬░C, substrate 4-methyldibenzothiophene-5,5-dioxide Paenibacillus sp.
22.4
-
purified enzyme, pH 7.0, 50┬░C, substrate 1-methyldibenzothiophene-5,5-dioxide Paenibacillus sp.
29.6
-
purified enzyme, pH 7.0, 50┬░C, substrate 3-methyldibenzothiophene-5,5-dioxide Paenibacillus sp.
37.2 40 purified enzyme, pH 7.0, 50┬░C, substrate dibenzothiophene-5,5-dioxide Paenibacillus sp.
38.4
-
purified enzyme, pH 7.0, 50┬░C, substrate 2-methyldibenzothiophene-5,5-dioxide Paenibacillus sp.
62.4
-
purified enzyme, pH 7.0, 50┬░C, substrate 3,4,6-trimethyldibenzothiophene-5,5-dioxide Paenibacillus sp.
77.2
-
purified enzyme, pH 7.0, 50┬░C, substrate 4,6-dimethyldibenzothiophene-5,5-dioxide Paenibacillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-methyldibenzothiophene-5,5-dioxide + 2 FMNH2 + O2
-
Paenibacillus sp. ?
-
?
1-methyldibenzothiophene-5,5-dioxide + 2 FMNH2 + O2
-
Paenibacillus sp. A11-2 ?
-
?
2-methyldibenzothiophene-5,5-dioxide + 2 FMNH2 + O2
-
Paenibacillus sp. ?
-
?
2-methyldibenzothiophene-5,5-dioxide + 2 FMNH2 + O2
-
Paenibacillus sp. A11-2 ?
-
?
3,4,6-trimethyldibenzothiophene-5,5-dioxide + 2 FMNH2 + O2
-
Paenibacillus sp. ?
-
?
3,4,6-trimethyldibenzothiophene-5,5-dioxide + 2 FMNH2 + O2
-
Paenibacillus sp. A11-2 ?
-
?
3,6-dimethyldibenzothiophene-5,5-dioxide + 2 FMNH2 + O2
-
Paenibacillus sp. ?
-
?
3,6-dimethyldibenzothiophene-5,5-dioxide + 2 FMNH2 + O2
-
Paenibacillus sp. A11-2 ?
-
?
3-methyldibenzothiophene-5,5-dioxide + 2 FMNH2 + O2
-
Paenibacillus sp. ?
-
?
4,6-dimethyldibenzothiophene-5,5-dioxide + 2 FMNH2 + O2
-
Paenibacillus sp. ?
-
?
4-methyldibenzothiophene-5,5-dioxide + 2 FMNH2 + O2
-
Paenibacillus sp. ?
-
?
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2
-
Paenibacillus sp. 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O
-
?
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2
-
Paenibacillus sp. A11-2 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 48000, SDS-PAGE Paenibacillus sp.

Synonyms

Synonyms Comment Organism
DBT sulfone monooxygenase
-
Paenibacillus sp.
dibenzothiophene sulfone monooxygenase
-
Paenibacillus sp.
TdsA
-
Paenibacillus sp.

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
45
-
-
Paenibacillus sp.

Temperature Range [┬░C]

Temperature Minimum [┬░C] Temperature Maximum [┬░C] Comment Organism
30 65 activity range, profile overview Paenibacillus sp.

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
60
-
purified enzyme, pH 7.0, highly stable up to Paenibacillus sp.
70
-
purified enzyme, pH 7.0, inactivation Paenibacillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
Paenibacillus sp.

Cofactor

Cofactor Comment Organism Structure
FMNH2
-
Paenibacillus sp.
additional information the purified TdsA absolutely requires an oxidoreductase for its activity, oxidoreductase TdsD is completely FMN-dependent, and FAD does not act as a cofactor. TsdD is active with NADH and NADPH to the same extent. TdsA exhibits no activity in the absence of TdsD Paenibacillus sp.