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Literature summary for 1.14.14.22 extracted from

  • Ishii, Y.; Konishi, J.; Okada, H.; Hirasawa, K.; Onaka, T.; Suzuki, M.
    Operon structure and functional analysis of the genes encoding thermophilic desulfurizing enzymes of Paenibacillus sp. A11-2 (2000), Biochem. Biophys. Res. Commun., 270, 81-88.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene tdsA, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant expression in Escherichia coli, coexpression with genes tdsC and tdsB, leading to established thermophilic desulfurization of dibenzothiophene in Escherichia coli host, an Escherichia coli oxidoreductase can be functionally coupled with the monooxygenases of a gram-positive thermophile. The end of tdsA shows a 4-bp overlap with the translational start site of tdsB Paenibacillus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38900
-
x * 38900, about, sequnence calculation Paenibacillus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 Paenibacillus sp.
-
2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O
-
?
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 Paenibacillus sp. A11-2
-
2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O
-
?

Organism

Organism UniProt Comment Textmining
Paenibacillus sp. Q9LBX4 gene tdsA
-
Paenibacillus sp. A11-2 Q9LBX4 gene tdsA
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2
-
Paenibacillus sp. 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O
-
?
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2
-
Paenibacillus sp. A11-2 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O
-
?

Subunits

Subunits Comment Organism
? x * 38900, about, sequnence calculation Paenibacillus sp.

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
37 50 assay at, cell extract Paenibacillus sp.

Cofactor

Cofactor Comment Organism Structure
FMNH2
-
Paenibacillus sp.