Cloned (Comment) | Organism |
---|---|
gene bdsC, recombinant overexpression in Escherichia coli | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Bacillus subtilis | |
2,2'-dithiobis(5-nitropyridine) | - |
Bacillus subtilis | |
4-chloromercuribenzoate | - |
Bacillus subtilis | |
8-Quinolinol | - |
Bacillus subtilis | |
Ag+ | - |
Bacillus subtilis | |
Cu2+ | - |
Bacillus subtilis | |
diethyldithiocarbamate | - |
Bacillus subtilis | |
Hg2+ | - |
Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
4 * 45000, SDS-PAGE | Bacillus subtilis |
200000 | - |
gel filtration | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dibenzothiophene + 2 FMNH2 + 2 O2 | Bacillus subtilis | - |
dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
dibenzothiophene + 2 FMNH2 + 2 O2 | Bacillus subtilis WU-S2B | - |
dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
gene bdsC | - |
Bacillus subtilis WU-S2B | - |
gene bdsC | - |
Purification (Comment) | Organism |
---|---|
native enzyme 19.2fold from strain WU-S2B by two different steps of anion exchange chromatography and one step of hydrophobic interaction chromatography, recombinant enzyme from Escherichia coli 4.5fold to homogeneity by anion exchange and hydrophobic interaction chromatography | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
84.2 | - |
purified native enzyme, pH 7.0, 50°C | Bacillus subtilis |
163.2 | - |
purified recombinant enzyme, pH 7.0, 50°C | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-methyldibenzothiophene + 2 FMNH2 + 2 O2 | - |
Bacillus subtilis | 1-methyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
2,8-dimethyldibenzothiophene + 2 FMNH2 + 2 O2 | - |
Bacillus subtilis | 2,8-dimethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
2,8-dimethyldibenzothiophene + 2 FMNH2 + 2 O2 | - |
Bacillus subtilis WU-S2B | 2,8-dimethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
2-methyldibenzothiophene + 2 FMNH2 + 2 O2 | - |
Bacillus subtilis | 2-methyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
3,4-benzo-dibenzothiophene + 2 FMNH2 + 2 O2 | - |
Bacillus subtilis | 3,4-benzo-dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
3-methyldibenzothiophene + 2 FMNH2 + 2 O2 | - |
Bacillus subtilis | 3-methyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
4,6-diethyldibenzothiophene + 2 FMNH2 + 2 O2 | - |
Bacillus subtilis | 4,6-diethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
4,6-dimethyldibenzothiophene + 2 FMNH2 + 2 O2 | - |
Bacillus subtilis | 4,6-dimethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
4,6-dimethyldibenzothiophene + 2 FMNH2 + 2 O2 | - |
Bacillus subtilis WU-S2B | 4,6-dimethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
4-methyldibenzothiophene + 2 FMNH2 + 2 O2 | - |
Bacillus subtilis | 4-methyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
4-methyldibenzothiophene + 2 FMNH2 + 2 O2 | - |
Bacillus subtilis WU-S2B | 4-methyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
dibenzothiophene + 2 FMNH2 + 2 O2 | - |
Bacillus subtilis | dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
dibenzothiophene + 2 FMNH2 + 2 O2 | - |
Bacillus subtilis WU-S2B | dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
additional information | coupled reaction with flavin reductase (TdsD) from thermophilic Paenibacillus sp. strain A11-2. Substrate specificity, overview. No activity with 4,6-di-n-propyl-DBT, 4,6-di-n-butyl-DBT, carbazole, dibenzofuran, fluorene, biphenyl, DBT sulfone, benzothiophene, and naphthothiophene | Bacillus subtilis | ? | - |
? | |
additional information | coupled reaction with flavin reductase (TdsD) from thermophilic Paenibacillus sp. strain A11-2. Substrate specificity, overview. No activity with 4,6-di-n-propyl-DBT, 4,6-di-n-butyl-DBT, carbazole, dibenzofuran, fluorene, biphenyl, DBT sulfone, benzothiophene, and naphthothiophene | Bacillus subtilis WU-S2B | ? | - |
? | |
thioxanthen-9-one + 2 FMNH2 + 2 O2 | - |
Bacillus subtilis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 45000, SDS-PAGE | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
BdsC | - |
Bacillus subtilis |
DBT monooxygenase | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
purified enzyme, 30 min, 80% activity remaning | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Bacillus subtilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | 11 | purified enzyme, stable at | Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMNH2 | - |
Bacillus subtilis |