Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.14.21 extracted from

  • Ohshiro, T.; Ishii, Y.; Matsubara, T.; Ueda, K.; Izumi, Y.; Kino, K.; Kirimura, K.
    Dibenzothiophene desulfurizing enzymes from moderately thermophilic bacterium Bacillus subtilis WU-S2B: purification, characterization and overexpression (2005), J. Biosci. Bioeng., 100, 266-273.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene bdsC, recombinant overexpression in Escherichia coli Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Bacillus subtilis
2,2'-dithiobis(5-nitropyridine)
-
Bacillus subtilis
4-chloromercuribenzoate
-
Bacillus subtilis
8-Quinolinol
-
Bacillus subtilis
Ag+
-
Bacillus subtilis
Cu2+
-
Bacillus subtilis
diethyldithiocarbamate
-
Bacillus subtilis
Hg2+
-
Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
4 * 45000, SDS-PAGE Bacillus subtilis
200000
-
gel filtration Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dibenzothiophene + 2 FMNH2 + 2 O2 Bacillus subtilis
-
dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
?
dibenzothiophene + 2 FMNH2 + 2 O2 Bacillus subtilis WU-S2B
-
dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
gene bdsC
-
Bacillus subtilis WU-S2B
-
gene bdsC
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 19.2fold from strain WU-S2B by two different steps of anion exchange chromatography and one step of hydrophobic interaction chromatography, recombinant enzyme from Escherichia coli 4.5fold to homogeneity by anion exchange and hydrophobic interaction chromatography Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
84.2
-
purified native enzyme, pH 7.0, 50°C Bacillus subtilis
163.2
-
purified recombinant enzyme, pH 7.0, 50°C Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-methyldibenzothiophene + 2 FMNH2 + 2 O2
-
Bacillus subtilis 1-methyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
?
2,8-dimethyldibenzothiophene + 2 FMNH2 + 2 O2
-
Bacillus subtilis 2,8-dimethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
?
2,8-dimethyldibenzothiophene + 2 FMNH2 + 2 O2
-
Bacillus subtilis WU-S2B 2,8-dimethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
?
2-methyldibenzothiophene + 2 FMNH2 + 2 O2
-
Bacillus subtilis 2-methyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
?
3,4-benzo-dibenzothiophene + 2 FMNH2 + 2 O2
-
Bacillus subtilis 3,4-benzo-dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
?
3-methyldibenzothiophene + 2 FMNH2 + 2 O2
-
Bacillus subtilis 3-methyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
?
4,6-diethyldibenzothiophene + 2 FMNH2 + 2 O2
-
Bacillus subtilis 4,6-diethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
?
4,6-dimethyldibenzothiophene + 2 FMNH2 + 2 O2
-
Bacillus subtilis 4,6-dimethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
?
4,6-dimethyldibenzothiophene + 2 FMNH2 + 2 O2
-
Bacillus subtilis WU-S2B 4,6-dimethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
?
4-methyldibenzothiophene + 2 FMNH2 + 2 O2
-
Bacillus subtilis 4-methyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
?
4-methyldibenzothiophene + 2 FMNH2 + 2 O2
-
Bacillus subtilis WU-S2B 4-methyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
?
dibenzothiophene + 2 FMNH2 + 2 O2
-
Bacillus subtilis dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
?
dibenzothiophene + 2 FMNH2 + 2 O2
-
Bacillus subtilis WU-S2B dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
?
additional information coupled reaction with flavin reductase (TdsD) from thermophilic Paenibacillus sp. strain A11-2. Substrate specificity, overview. No activity with 4,6-di-n-propyl-DBT, 4,6-di-n-butyl-DBT, carbazole, dibenzofuran, fluorene, biphenyl, DBT sulfone, benzothiophene, and naphthothiophene Bacillus subtilis ?
-
?
additional information coupled reaction with flavin reductase (TdsD) from thermophilic Paenibacillus sp. strain A11-2. Substrate specificity, overview. No activity with 4,6-di-n-propyl-DBT, 4,6-di-n-butyl-DBT, carbazole, dibenzofuran, fluorene, biphenyl, DBT sulfone, benzothiophene, and naphthothiophene Bacillus subtilis WU-S2B ?
-
?
thioxanthen-9-one + 2 FMNH2 + 2 O2
-
Bacillus subtilis ?
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 45000, SDS-PAGE Bacillus subtilis

Synonyms

Synonyms Comment Organism
BdsC
-
Bacillus subtilis
DBT monooxygenase
-
Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
purified enzyme, 30 min, 80% activity remaning Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Bacillus subtilis

pH Stability

pH Stability pH Stability Maximum Comment Organism
8 11 purified enzyme, stable at Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
FMNH2
-
Bacillus subtilis