Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.14.21 extracted from

  • Duan, X.; Zhang, L.; Zhou, D.; Ji, K.; Ma, T.; Shui, W.; Li, G.; Li, X.
    Crystallization and preliminary structural analysis of dibenzothiophene monooxygenase (DszC) from Rhodococcus erythropolis (2013), Acta Crystallogr. Sect. F, 69, 597-601.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene dszC, cloning from strain DS-3, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) and of selenomethionine-labeled enzyme in Escherichia coli strain B834(DE3) Rhodococcus erythropolis

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant wild-type and selenomethionine-labeled enzymes, hanging-drop vapour-diffusion method, mixing of 10 mg/ml wild-type enzyme in 20 mM Tris-HCl, pH 8.0, 150 mM NaCl with reservoir solution containing 17.5% PEG 3350, 200 mM Bis-Tris, pH 7.5, and 200 mM ammonium sulfate, and mixing of 5 mg/ml selenomethionine-labeled enzyme in 20 mM Tris-HCl, pH 8.0, 150 mM NaCl with reservoir solution containing 25% PEG 3350, 100 mM PIPES, pH 7.0, 200 mM ammonium sulfate, 20°C, X-ray diffraction structure determination and analysis at 2.4-2.9 A resolution Rhodococcus erythropolis

Organism

Organism UniProt Comment Textmining
Rhodococcus erythropolis A0A0C6DRW4 i.e. D-1, gene dszC
-
Rhodococcus erythropolis DS-3 A0A0C6DRW4 i.e. D-1, gene dszC
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His-tagged enzyms from Escherichia coli strain by nickel affinity and anion exchange chromatography, and gel filtration Rhodococcus erythropolis

Synonyms

Synonyms Comment Organism
dibenzothiophene monooxygenase
-
Rhodococcus erythropolis
dszC
-
Rhodococcus erythropolis