Cloned (Comment) | Organism |
---|---|
gene dszC, cloning from strain DS-3, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) and of selenomethionine-labeled enzyme in Escherichia coli strain B834(DE3) | Rhodococcus erythropolis |
Crystallization (Comment) | Organism |
---|---|
purified recombinant wild-type and selenomethionine-labeled enzymes, hanging-drop vapour-diffusion method, mixing of 10 mg/ml wild-type enzyme in 20 mM Tris-HCl, pH 8.0, 150 mM NaCl with reservoir solution containing 17.5% PEG 3350, 200 mM Bis-Tris, pH 7.5, and 200 mM ammonium sulfate, and mixing of 5 mg/ml selenomethionine-labeled enzyme in 20 mM Tris-HCl, pH 8.0, 150 mM NaCl with reservoir solution containing 25% PEG 3350, 100 mM PIPES, pH 7.0, 200 mM ammonium sulfate, 20°C, X-ray diffraction structure determination and analysis at 2.4-2.9 A resolution | Rhodococcus erythropolis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus erythropolis | A0A0C6DRW4 | i.e. D-1, gene dszC | - |
Rhodococcus erythropolis DS-3 | A0A0C6DRW4 | i.e. D-1, gene dszC | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His-tagged enzyms from Escherichia coli strain by nickel affinity and anion exchange chromatography, and gel filtration | Rhodococcus erythropolis |
Synonyms | Comment | Organism |
---|---|---|
dibenzothiophene monooxygenase | - |
Rhodococcus erythropolis |
dszC | - |
Rhodococcus erythropolis |