Literature summary for 1.14.14.180 extracted from

Nomura, T.; Kushiro, T.; Yokota, T.; Kamiya, Y.; Bishop, G.J.; Yamaguchi, S.
The last reaction producing brassinolide is catalyzed by cytochrome P-450s, CYP85A3 in tomato and CYP85A2 in Arabidopsis (2005), J. Biol. Chem., 280, 17873-17879 .

Search for more literature for 1.14.14.180

Cloned(Commentary)

Cloned (Comment)	Organism
expression in yeast	Solanum lycopersicum

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q940V4	cf. EC 1.14.14.179, bifunctional brassinosteroid 6-oxygenase and brassinolide synthase	-
Solanum lycopersicum	Q50LE0	cf. EC 1.14.14.179, bifunctional brassinosteroid 6-oxygenase and brassinolide synthase	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
fruit	preferential expression	Solanum lycopersicum	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
castasterone + O2 + [reduced NADPH-hemoprotein reductase]	-	Arabidopsis thaliana	brassinolide + 2 H2O + [oxidized NADPH-hemoprotein reductase]	-	?
castasterone + O2 + [reduced NADPH-hemoprotein reductase]	-	Solanum lycopersicum	brassinolide + 2 H2O + [oxidized NADPH-hemoprotein reductase]	-	?

Synonyms

Synonyms	Comment	Organism
CYP85A2	-	Arabidopsis thaliana
CYP85A3	-	Solanum lycopersicum

General Information

General Information	Comment	Organism
evolution	in a phylogenetic tree, tomato CYP85A3 is placed on the same branch as the CYP85A family of tomato, Arabidopsis, and rice, and is closest to tomato CYP85A1	Solanum lycopersicum
physiological function	CYP85A2 shows castasterone synthase and also brassinolide synthase activity. Castasterone is the major active brassinosteroid during vegetative growth in tomato, whereas brassinolide may play an organ-specific role in fruit development. CYP85A2 T-DNA insertion mutants consistently exhibit a weak dwarf phenotype during early vegetative development and a reduced fertility after bolting. CYP85A1/CYP85A2 double mutants grow as severe dwarfs	Arabidopsis thaliana

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Release 2023.1 (January 2023)