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Literature summary for 1.14.14.18 extracted from

  • Spencer, A.L.; Bagai, I.; Becker, D.F.; Zuiderweg, E.R.; Ragsdale, S.W.
    Protein/protein interactions in the mammalian heme degradation pathway heme oxygenase-2, cytochrome P450 reductase, and biliverdin reductase (2014), J. Biol. Chem., 289, 29836-29858 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.14.18

Protein Variants

Protein Variants Comment Organism
K149 42fold increase in Km values for cytochrome P450 reductase Homo sapiens
K169A 22fold increase in Km values for cytochrome P450 reductase Homo sapiens
L201A 3fold increase in Km values for cytochrome P450 reductase Homo sapiens
R87A 1.3fold increase in Km values for cytochrome P450 reductase Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
 [reduced cytochrome P450 reductase] wild-type, pH 8.0, 37°C Homo sapiens
0.7
-
 [reduced cytochrome P450 reductase] wild-type, pH 8.0, 37°C Homo sapiens
0.9
-
 [reduced cytochrome P450 reductase] mutant R87A, pH 8.0, 37°C Homo sapiens
2.3
-
 [reduced cytochrome P450 reductase] mutant L201A, pH 8.0, 37°C Homo sapiens
15.2
-
 [reduced cytochrome P450 reductase] mutant K169A, pH 8.0, 37°C Homo sapiens
21.7
-
 [reduced cytochrome P450 reductase] mutant K149A, pH 8.0, 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P09601
-
-
Homo sapiens P30519
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
protoheme + [reduced cytochrome P450 reductase] + O2
-
 Homo sapiens biliverdin + Fe2+ + CO + [oxidized cytochrome P450 reductase] + H2O
-
 ?

General Information

General Information Comment Organism
physiological function Hmox2 and cytochrome P450 reductase form a dynamic ensemble of complexes that precede formation of the productive electron transfer complex. Specific residues, including Leu201, near the heme face of Hmox2 are affected by the addition of cytochrome P450 reductase. Hmox2 and biliverdin reductase form a very weak complex, and biliverdin reductase binds Hmox2 weakly Homo sapiens