Literature summary for 1.14.14.18 extracted from

Gisk, B.; Yasui, Y.; Kohchi, T.; Frankenberg-Dinkel, N.
Characterization of the heme oxygenase protein family in Arabidopsis thaliana reveals a diversity of functions (2010), Biochem. J., 425, 425-434.

Search for more literature for 1.14.14.18

Activating Compound

Activating Compound	Comment	Organism	Structure
ascorbate	addition of a second electron donor like ascorbate leads to 10times faster heme conversion	Arabidopsis thaliana
additional information	heme turnover is promoted by light when spinach thylakoids are present	Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment)	Organism
HY1, HO3, and HO4 expressed without their predicted chloroplast transit peptides either using a tac promoter-driven N-terminal GST fusion expression system (HY1) or the pET vector system (HO3, HO4) using a T7-promoter and a His-tag fusion. Plasmids pGEX-mHY1, pET24a-mHO3 and pET24a-mHO4 expressed in Escherichia coli BL21 (DE3). Fluorescent protein expression in Nicotiana benthamiana by using Agrobacterium tumefaciens C58C1 pGV2260	Arabidopsis thaliana

Cloned (Comment)

Organism

HY1, HO3, and HO4 expressed without their predicted chloroplast transit peptides either using a tac promoter-driven N-terminal GST fusion expression system (HY1) or the pET vector system (HO3, HO4) using a T7-promoter and a His-tag fusion. Plasmids pGEX-mHY1, pET24a-mHO3 and pET24a-mHO4 expressed in Escherichia coli BL21 (DE3). Fluorescent protein expression in Nicotiana benthamiana by using Agrobacterium tumefaciens C58C1 pGV2260

Arabidopsis thaliana

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0027	-	heme	HO3, without ascorbate	Arabidopsis thaliana
0.0057	-	heme	HO4, without ascorbate	Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
chloroplast	HY1, HO3, and HO4 are present as the processed mature protein in the plastid compartment	Arabidopsis thaliana	9507	-
additional information	no mitochondrial localization of HY1, HO3, and HO4	Arabidopsis thaliana	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
27000	-	1 * 27000, HY1, SDS-PAGE. HY1, HO3 and HO4, gel filtration	Arabidopsis thaliana

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
HY1, HO3, and HO4. HO3 and HO4 purified by cobalt affinity chromatography, to over 90% purity	Arabidopsis thaliana

Storage Stability

Storage Stability	Organism
0°C, 100 mM potassium phosphate buffer, pH 7.2, 4 days	Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
heme + ferredoxin + O2	HY1, HO3, and HO4	Arabidopsis thaliana	biliverdin IXalpha + Fe2+ + CO + A + H2O	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 27000, HY1, SDS-PAGE. HY1, HO3 and HO4, gel filtration	Arabidopsis thaliana

Synonyms

Synonyms	Comment	Organism
Ho3	-	Arabidopsis thaliana
HO4	-	Arabidopsis thaliana
HY1	HO1	Arabidopsis thaliana

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
10	45	-	Arabidopsis thaliana

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	HO3	Arabidopsis thaliana
7.25	-	HY1 and HO4	Arabidopsis thaliana

Cofactor

Cofactor	Comment	Organism	Structure
heme	-	Arabidopsis thaliana
NADPH	-	Arabidopsis thaliana