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Literature summary for 1.14.14.18 extracted from

  • Trakshel, G.M.; Kutty, R.K.; Maines, M.D.
    Purification and characterization of the major constitutive form of testicular heme oxygenase. The noninducible isoform (1986), J. Biol. Chem., 261, 11131-11137.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
CO
-
Rattus norvegicus
KCN
-
Rattus norvegicus
NaN3
-
Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
x * 30000, liver heme oxygenase-1, SDS-PAGE Rattus norvegicus
36000
-
x * 36000, testis heme oxygenase-2, SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
2 isoforms: heme oxygenase-1, inducible
-
Rattus norvegicus
-
heme oxygenase-2, constitutive
-
Rattus norvegicus
-
only minute amounts of heme oxygenase-1 in testis
-

Purification (Commentary)

Purification (Comment) Organism
heme oxygenase-2 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
testis
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.067
-
testicular heme oxygenase-2 Rattus norvegicus
0.11
-
liver heme oxygenase-1 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
heme + electron donor + O2 overview, substrate specificity Rattus norvegicus biliverdin + Fe2+ + CO + oxidized eletron donor + H2O
-
?
heme + electron donor + O2 testis heme oxygenase 2 oxidizes Fe-protopophyrin, ferric hematoporphyrin acetate and ferric hematoporphyrin Rattus norvegicus biliverdin + Fe2+ + CO + oxidized eletron donor + H2O
-
?
heme + electron donor + O2 NADPH is more effective than NADH Rattus norvegicus biliverdin + Fe2+ + CO + oxidized eletron donor + H2O
-
?

Subunits

Subunits Comment Organism
? x * 30000, liver heme oxygenase-1, SDS-PAGE Rattus norvegicus
? x * 36000, testis heme oxygenase-2, SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
liver heme oxygenase-1: 65-70% activity after 10 min at 60°C, testis heme oxygenase-2: 20% activity after 10 min at 60°C Rattus norvegicus
65
-
10 min, 30% loss of heme oxygenase-1 activity, 80% loss of heme oxygenase-2 activity Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
liver heme oxygenase-1 and testis heme oxygenase-2 Rattus norvegicus