Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CO | - |
Rattus norvegicus | |
KCN | - |
Rattus norvegicus | |
NaN3 | - |
Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
x * 30000, liver heme oxygenase-1, SDS-PAGE | Rattus norvegicus |
36000 | - |
x * 36000, testis heme oxygenase-2, SDS-PAGE | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
2 isoforms: heme oxygenase-1, inducible | - |
Rattus norvegicus | - |
heme oxygenase-2, constitutive | - |
Rattus norvegicus | - |
only minute amounts of heme oxygenase-1 in testis | - |
Purification (Comment) | Organism |
---|---|
heme oxygenase-2 | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
testis | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.067 | - |
testicular heme oxygenase-2 | Rattus norvegicus |
0.11 | - |
liver heme oxygenase-1 | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
heme + electron donor + O2 | overview, substrate specificity | Rattus norvegicus | biliverdin + Fe2+ + CO + oxidized eletron donor + H2O | - |
? | |
heme + electron donor + O2 | testis heme oxygenase 2 oxidizes Fe-protopophyrin, ferric hematoporphyrin acetate and ferric hematoporphyrin | Rattus norvegicus | biliverdin + Fe2+ + CO + oxidized eletron donor + H2O | - |
? | |
heme + electron donor + O2 | NADPH is more effective than NADH | Rattus norvegicus | biliverdin + Fe2+ + CO + oxidized eletron donor + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30000, liver heme oxygenase-1, SDS-PAGE | Rattus norvegicus |
? | x * 36000, testis heme oxygenase-2, SDS-PAGE | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
liver heme oxygenase-1: 65-70% activity after 10 min at 60°C, testis heme oxygenase-2: 20% activity after 10 min at 60°C | Rattus norvegicus |
65 | - |
10 min, 30% loss of heme oxygenase-1 activity, 80% loss of heme oxygenase-2 activity | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
liver heme oxygenase-1 and testis heme oxygenase-2 | Rattus norvegicus |