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Literature summary for 1.14.14.18 extracted from

  • Maines, M.D.; Ibrahim, N.G.; Kappas, A.
    Solubilization and partial purification of heme oxygenase from rat liver (1977), J. Biol. Chem., 252, 5900-5903.
    View publication on PubMed
Search for more literature for 1.14.14.18

Activating Compound

Activating Compound Comment Organism Structure
cysteine 0.1 mM, slight stimulation, inhibition at 1 mM Rattus norvegicus

General Stability

General Stability Organism
50% loss of activity during one cycle of freezing and thawing Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 0.1 mM, 81% inhibition Rattus norvegicus
4-hydroxymercuribenzoate 1 mM, complete inhibition Rattus norvegicus
cysteine 1 mM, 67% inhibition Rattus norvegicus
dithiothreitol 0.01 mM, 88% inhibition Rattus norvegicus
Hg2+ 0.3 mM, complete inhibition Rattus norvegicus
iodoacetamide 5 mM, 39% inhibition, 10 mM, 79% inhibition Rattus norvegicus
p-chloromercuribenzoate
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 heme
-
 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Rattus norvegicus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
 x * 68000, possibly a trimer, SDS-PAGE Rattus norvegicus
180000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
heme + 2 NADPH + 2 H+ + 2 O2 Rattus norvegicus involved in heme metabolism biliverdin + Fe2+ + CO + 2 NADP+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-cellulose, Sephadex G-200 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12.7
-
-
 Rattus norvegicus

Storage Stability

Storage Stability Organism
-20°C, 2 weeks Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
heme + 2 NADPH + 2 H+ + 2 O2 involved in heme metabolism Rattus norvegicus biliverdin + Fe2+ + CO + 2 NADP+ + H2O
-
 ?
heme + electron donor + O2 electron donor NADH Rattus norvegicus biliverdin + Fe2+ + CO + oxidized eletron donor + H2O
-
 ?
heme + electron donor + O2 NADPH is more effective than NADH Rattus norvegicus biliverdin + Fe2+ + CO + oxidized eletron donor + H2O
-
 ?
heme + electron donor + O2 electron donor NADPH Rattus norvegicus biliverdin + Fe2+ + CO + oxidized eletron donor + H2O
-
 ?
methemoglobin + electron donor + O2 30% of activity with heme Rattus norvegicus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 68000, possibly a trimer, SDS-PAGE Rattus norvegicus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
 no activity at 0°C Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 5 min, complete loss of activity Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NADH NADPH is more effective than NADH Rattus norvegicus
NADPH NADPH is more effective than NADH Rattus norvegicus