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Literature summary for 1.14.14.173 extracted from

  • Fang, L.; Qin, H.; Shi, T.; Wu, X.; Li, Q.; Hua, R.
    Ortho and para oxydehalogenation of dihalophenols catalyzed by the monooxygenase TcpA and NAD(P)H FAD reductase Fre (2020), J. Hazard. Mater., 388, 121787 .
    View publication on PubMed
Search for more literature for 1.14.14.173

Cloned(Commentary)

Cloned (Comment) Organism
enzyme fused to maltose-binding protein, expressed in Escherichia coli BL21(DE3) cells Cupriavidus nantongensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,4,6-trichlorophenol + FADH2 + O2 Cupriavidus nantongensis
-
 2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O
-
 ?
2,4,6-trichlorophenol + FADH2 + O2 Cupriavidus nantongensis X1
-
 2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O
-
 ?
2,4,6-trichlorophenol + FADH2 + O2 Cupriavidus nantongensis X1T
-
 2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O
-
 ?
2,6-dichlorohydroquinone + FADH2 + O2 Cupriavidus nantongensis
-
 2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O
-
 ?
2,6-dichlorohydroquinone + FADH2 + O2 Cupriavidus nantongensis X1
-
 2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O
-
 ?
2,6-dichlorohydroquinone + FADH2 + O2 Cupriavidus nantongensis X1T
-
 2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Cupriavidus nantongensis
-
-
-
Cupriavidus nantongensis X1T
-
-
-

Purification (Commentary)

Purification (Comment) Organism
amylose resin column chromatography and Ni-NTA column chromatography Cupriavidus nantongensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3,4,5-tetrachlorophenol + FADH2 + O2 73% activity compared to 2,4-dichlorophenol Cupriavidus nantongensis ?
-
 ?
2,3,4,5-tetrachlorophenol + FADH2 + O2 73% activity compared to 2,4-dichlorophenol Cupriavidus nantongensis X1 ?
-
 ?
2,3,4,5-tetrachlorophenol + FADH2 + O2 73% activity compared to 2,4-dichlorophenol Cupriavidus nantongensis X1T ?
-
 ?
2,3,4,6-tetrachlorophenol + FADH2 + O2 80% activity compared to 2,4-dichlorophenol Cupriavidus nantongensis ?
-
 ?
2,3-dichlorophenol + FADH2 + O2
-
 Cupriavidus nantongensis 2,3-dichlorohydroquinone + FAD + H2O
-
 ?
2,4,5-trichlorophenol + FADH2 + O2 87% activity compared to 2,4-dichlorophenol Cupriavidus nantongensis ?
-
 ?
2,4,6-trichlorophenol + FADH2 + O2
-
 Cupriavidus nantongensis 2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O
-
 ?
2,4,6-trichlorophenol + FADH2 + O2
-
 Cupriavidus nantongensis X1 2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O
-
 ?
2,4,6-trichlorophenol + FADH2 + O2
-
 Cupriavidus nantongensis X1T 2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O
-
 ?
2,4,6-trichlorophenol + FADH2 + O2 94% activity compared to 2,4-dichlorophenol Cupriavidus nantongensis ?
-
 ?
2,4-dichlorophenol + FADH2 + O2 100% activity, 96.73% degradation in 24 h Cupriavidus nantongensis 2-chlorobenzoquinone + Cl- + FAD + H2O
-
 ?
2,5-dichlorophenol + FADH2 + O2
-
 Cupriavidus nantongensis 2,5-dichlorohydroquinone + FAD + H2O
-
 ?
2,6-dichlorohydroquinone + FADH2 + O2
-
 Cupriavidus nantongensis 2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O
-
 ?
2,6-dichlorohydroquinone + FADH2 + O2
-
 Cupriavidus nantongensis X1 2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O
-
 ?
2,6-dichlorohydroquinone + FADH2 + O2
-
 Cupriavidus nantongensis X1T 2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O
-
 ?
2,6-dichlorophenol + FADH2 + O2
-
 Cupriavidus nantongensis 2,6-dichlorohydroquinone + FAD + H2O
-
 ?
2-bromo-4-chlorophenol + FADH2 + O2 87.31% degradation in 24 h Cupriavidus nantongensis 2-bromobenzoquinone + Cl- + FAD + H2O
-
 ?
2-chloro-4-bromophenol + FADH2 + O2 74.48% degradation in 24 h Cupriavidus nantongensis 2-chlorobenzoquinone + Br- + FAD + H2O
-
 ?
2-chloro-4-nitrophenol + FADH2 + O2 98.37% degradation in 24 h Cupriavidus nantongensis 2-chlorobenzoquinone + NO2- + FAD + H2O
-
 ?
3,4-dichlorophenol + FADH2 + O2
-
 Cupriavidus nantongensis 3-chlorobenzoquinone + Cl- + FAD + H2O
-
 ?
3,5-dichlorophenol + FADH2 + O2
-
 Cupriavidus nantongensis 3,5-dichlorohydroquinone + FAD + H2O
-
 ?
additional information no activity with 2-chloro-4-methylphenol and 2-chlorohydroquinone Cupriavidus nantongensis ?
-
-
additional information no activity with 2-chloro-4-methylphenol and 2-chlorohydroquinone Cupriavidus nantongensis X1 ?
-
-
additional information no activity with 2-chloro-4-methylphenol and 2-chlorohydroquinone Cupriavidus nantongensis X1T ?
-
-

Subunits

Subunits Comment Organism
homotetramer
-
 Cupriavidus nantongensis

Synonyms

Synonyms Comment Organism
tcpA
-
 Cupriavidus nantongensis

Cofactor

Cofactor Comment Organism Structure
FADH2
-
 Cupriavidus nantongensis
NADH
-
 Cupriavidus nantongensis

General Information

General Information Comment Organism
metabolism the enzyme has dual dehalogenation and denitration functions Cupriavidus nantongensis